Development of a peptide array synthesis system

描述（由申请人提供）：本提案第一阶段和第二阶段的总体目标是开发基于微阵列的方法，用于高通量蛋白质分析。这将以两种不同的方式完成，一种是基于在1平方厘米的芯片上成千上万个不同的肽的阵列，可以用来识别蛋白质，另一种是基于数千或数万个针对蛋白质的抗体，只给出蛋白质的基因组序列。这两种方法都需要一种低成本、快速生产高密度肽阵列的制造设备。具体而言，第一阶段的目标如下：合成光敏氨基酸将光敏氨基酸附着在氨基玻璃载玻片上。3. 确定最佳脱保护时间和合适的溶剂条件。提高氨基酸偶联每步产率。4. 合成肽阵列。5. 在已知肽蛋白相互作用的小范围内演示系统，建立信号可重复性和响应特性。6. 发展硅方法，以有效地选择数以百万计的不同的肽序列，这样几十个不同的序列将结合几乎任何蛋白质呈现到阵列。

DESCRIPTION (provided by applicant): The overall goal of phase I and II of this proposal is to develop microarray-based methods for high throughput profiling of proteins. This will be done in two different ways, one based on arrays of 100s of thousands of distinct peptides on 1 cm2 chips that can be used to fingerprint proteins, the other based on thousands or tens of thousands of antibodies against proteins, given only the genomic sequence of the proteins. Both methods require a fabricator for producing high density peptide arrays inexpensively and rapidly. In particular, phase I has the following aims: 1. Synthesize photolabile- amino acids 2. Attach photolabile amino acids to amino-glass slide. 3. Determine optimum deprotection time and appropriate solvent conditions. Improve the yield per step of amino acid coupling. 4. Synthesize peptide arrays. 5. Demonstrate the systems on a small scale for known peptide protein interactions, establishing signal reproducibility and response characteristics. 6. Develop in silico methods to effectively select millions of different peptide sequences such that tens of different sequences will bind virtually any protein presented to the array.