Animal Resources and Pathology Core

这一核心对于提供用于进行活体实验的最佳兽医护理和设施至关重要。该项目的S将获得比较医学部AAALAC的认可，集中管理和运营125,000平方米。英国《金融时报》该中心位于研究所的五栋建筑中。在过去的八年里，每一个单元都已经建成或进行了大规模的翻新。这些建筑中有四个有三个走廊系统，三个有专门的生物遏制装置，用于传染病或危险的化学实验。也有用于容纳无特定病原体的转基因小鼠群体的屏障设施。这些动物设施为麻省理工学院的研究人员提供了一个受控的环境，提供了必要的空间、设备和密封设施，用于在动物身上进行生物医学相关研究，包括细菌逃逸工作，并确保了实验过程中的人员安全。DCM比较病理学实验室还将提供人员和专业知识，对在计划项目框架内进行的活体研究提供的动物组织进行组织学和免疫细胞化学分析。具体地说，核心2的作用是提供设施、设备和人员，以集中管理和监督畜牧业和实验动物操作，并提供组织病理学和转基因服务，涉及本计划项目赠款中进行的研究。具体目标如下： 具体目标#1.通过胚胎移植重新获得转基因和其他特殊小鼠品系，以确保本项目中使用的GEM无特定病原体状态 具体目标#2.提供GEM的群体管理和疾病预防，供项目PI使用，并根据需要通过DNA原核显微注射或胚胎干细胞操作产生转基因动物 具体目标#3.提供组织学、病理学和分子特征方面的专业知识，为本项目中使用的小鼠模型的广泛表型和病理损伤评估提供依据。

This Core is essential for the delivery of optimal veterinary care and facilities utilized for the conduct of in vivo experimentation. The PI.'s of this program project will have access to the Division of Comparative Medicine's AAALAC accredited, centrally managed and operated 125,000 sq. ft. of space, located in five buildings at the Institute. Each of the units has been built or extensively renovated in the last eight years. Four of these buildings have three corridor systems and three have specialized bio-containment units for infectious or hazardous chemical experimentation. Barrier facilities for housing specific pathogen free transgenic mouse colonies are also available. These animal facilities provide MIT investigators a controlled environment with the necessary space, equipment, and containment facilities for performing biomedical related research in animals including germ-flee work and also ensures personnel safety during the experimental process. The DCM Comparative Pathology Laboratory will also provide the personnel and expertise to perform histological and immunocytochemical analysis of animal tissues delivered from in vivo studies performed within the framework of the program project.Specifically the role of Core 2 is to provide facilities, equipment, and personnel with expertise to centrally manage and supervise animal husbandry and experimental animal manipulations and provide histopathologic and transgenic services involved in studies conducted in this Program Project Grant. The specific aims are as follows: Specific aim #1. To rederive transgenic and other specialized mouse strains by embryo transfer to assure specific pathogen free status of GEM used in this program Specific aim #2. Provide colony management and disease prevention of GEM for use by program project PI's and as needed generate transgenic animals by pronuclear microinjection of DNA constructs or embryonic stem cell manipulation Specific aim #3. To provide histology, pathology and molecular characterization expertise for extensive phenotyping and assessment of pathological damage of mouse models used in this program.