CORE--ISLET PRODUCTION

描述(由申请人提供)： 该核心的主要目标是为加州大学旧金山分校的DRTC社区制作和提供 小鼠、非人灵长类(NHP)和人类供体的胰腺组织和纯化的胰岛 胰腺。我们的重点是为每位研究人员提供尽可能好的胰腺组织和胰岛，确保这些样本的质量和重复性。我们将专注于开发新的技术，旨在最大限度地减少胰岛细胞死亡，最大化胰岛产量和优化胰岛功能。我们将与胰岛、b细胞领域的专家密切合作，确定有助于我们实现更好的新胰岛分离技术目标的因素。Core将继续开发分析方法，以更好地评估胰岛制剂的质量。研究更多信息量更大的质量控制方法，这些方法将前瞻性地预测活体胰岛功能，而不是目前所依赖的有限的前瞻性研究。Core将与我们提供胰腺组织和胰岛的基础科学家合作，帮助我们充分利用胰岛细胞扩增和新生细胞进入大规模胰岛移植环境的新研究。核心还将维护最先进的人类胰岛隔离设施 符合FDA关于人胰岛分离和移植的所有cGMP规定。核心中心还将维护NHP和啮齿动物胰岛隔离设施。该核心将集中于将人类胰岛分离实验室中开发的积极方面，并将这些技术和分析应用于NHP和啮齿动物设施，反之亦然。胰岛生产设施核心将在DRTC的实验和临床计划中发挥关键作用，我们的目标是提供尽可能好的组织，并帮助推进胰岛和β细胞的研究。

DESCRIPTION (provided by applicant): The major objectives of this core are to produce and provide, to the UCSF DRTC community, pancreatic tissues and purified islets from mouse, non-human primates (NHP), and human donor pancreas. Our focus is to provide each investigator the best possible pancreatic tissue and islets, ensuring the quality and reproducibility of these samples. We will focus on developing new techniques that aim to minimize islet cell death, maximize islet yields and optimize islet function. We will work closely with experts in the islet, b-cell field to identify factors that will contribute to us reaching our goals for better new islet isolation techniques. The Core will continue to develop assays to better assess the quality of a pancreatic islet preparation. Investigate more informative quality control assays, which will prospectively predict in vivo islet function rather than the limited prospective studies relied upon today. The Core will work with the basic scientists, which we provide pancreas tissue and islets, to help us take full advantage of new research in islet cell expansion and neogenesis into large-scale islet transplantation settings. The Core will also maintain the state-of-the-art human islet isolation facilities meeting all FDA's cGMP regulations for human islet isolation and transplantation. The Core will also maintain the NHP and rodent pancreatic islet isolation facilities. The Core will focus on taking the positive aspects to what has been developed in the human islet isolation laboratory and apply these techniques and assays to both the NHP and rodent facilities and vise versa. The Islet Production Facility Core will play a critical role in the experimental and clinical programs of the DRTC and our objective is to provide the best possible tissues and to aid in the advancement of islet and beta-cells research.