Molecular And Immunopathology Of Experimental And Clinic

通过常规病理学、免疫组织化学、原位杂交、显微解剖和各种分子技术（包括PCR、RT-PCR和SNP）分析患者标本中眼部炎症细胞、退行性细胞和肿瘤细胞及其产物的身份和地形定位。尖端病理技术的应用使我们能够对疾病进行准确的病理评估，指导我们为患者选择合适的治疗方法，更好地了解疾病机制。在2002财年，我们继续并完成了以下研究：眼部淋巴瘤细胞中基因和蛋白的检测：我们继续检测IgH基因重排和玻璃体IL-10升高，作为原发性眼内b细胞淋巴瘤（PIOL）诊断的有用辅助手段。我们在RPE细胞上发现了b细胞趋化因子的蛋白和信使，在PIOL细胞上发现了b细胞趋化因子受体，这可能是PIOL在Bruch膜和RPE之间定位的机制，这提示了针对特异性趋化因子治疗PIOL的可能性。2. 眼部炎症及其他疾病的新病理及发病机制：利用显微解剖和PCR技术，我们能够检测出多种与艾滋病相关的眼部病原体，并诊断出引起眼内炎的是痤疮丙酸杆菌。我们是第一个报道畸胎瘤相关的副瘤性视网膜病变、先天性眼球震颤患者眼外肌肌腱束束神经解剖异常以及Rieger综合征先天性虹膜角膜畸形的独特病理的研究小组。3. 各种炎症性眼病的实验模型：我们继续研究炎症介质和细胞因子在内毒素诱导的葡萄膜炎模型（EIU）中的作用。在与博士合作。Caspi和Gery，开发并发表了几种新的眼部炎症模式。

The identity and topographic localization of ocular inflammatory, degenerative, and tumor cells and their products in patient specimens are analyzed by routine pathology, immunohistochemistry, in situ hybridization, microdissection and various molecular techniques including PCR, RT-PCR, and SNP. The application of cutting-edged pathological technology allows us to provide accurate pathological assessments of the disease, to guide us to select an appropriate therapy for the patient, and to better understand disease mechanism. In FY2002 we have continued and accomplished the following research: 1. Detection of Genes and Proteins in Ocular Lymphoma Cells: We continue to detect IgH gene rearrangemnts and vitreal IL-10 elevations being a useful adjunct for the diagnosis of primary intraocular B-cell lymphoma (PIOL). We found both the proteins and messengers of B-cell chemokines on RPE cells and the B-cell chemokine receptors on the PIOL cells, a possible mechanism for the PIOL localization between Bruch membrane and the RPE in the eye, which suggests targeting specific chemokines to treat PIOL. 2. New Pathology and Pathogenesis of Ocular Inflammation and Other Diseases: Using microdissection and PCR, we are able to detect various ocular pathogens associated with AIDS and diagnosed Propionibacterium acnes as causing endophthalmitis. We were the first group who reported teratoma-associated paraneoplastic retinopathy, abnormal neuroanatomy of the extraocular muscle tendon enthesis in patients with congenital nystagmus, and a unique pathology of congenital iridocorneal malformation in Rieger syndrome. 3. Experimental Models for Various Inflammatory Ocular Diseases: We continue to investigate the role of inflammatory mediators and cytokines in endotoxin-induced uveitis model (EIU). In collaboration with Drs. Caspi and Gery, several new modes of ocular inflammation were developed and published.