Synthesis and Study of Novel Sensing Agents

描述：（申请人的描述）本提案解决了三个重要问题 生物医学科学中的一个问题，通过开发简单和快速的检测方法 for biomecules生物分子.第一个目标是开发一种室温 果糖的显色测定，果糖是一种普遍存在的快速代谢的糖。的 所提出的测定被设计为在大的果糖存在下具有果糖选择性。 过量的葡萄糖。血浆中果糖的测定目前受到阻碍， 大量过量的葡萄糖第二个目标是创造一种温和的检测方法第二个目标是创造一种温和的检测方法 唾液酸是一种重要的细胞表面抗原， 基于水解程序和/或相对 非特定和苛刻的方法。第三个目标需要执行一项 新的低聚糖自动颜色检测系统， 先进行破坏性水解或用发色团进行化学标记。目前 对于中性寡糖，不存在有效的检测技术 用于目视检测超过三个重复单元的寡糖。

DESCRIPTION: (Applicant's Description) This proposal addresses three important problem in biomedical science via the development of simple and rapid assays for biomolecules. The first goal involves the development of a room temperature color assay for fructose, a ubiquitous, rapidly metabolized sugar. The proposedassay is designed to be fructose-selective in the presence of a large excess of glucose. Fructose determination in plasma is currently hampered due to the large excess of glucose. The second goal involves creatinga mild assay for sialic acid, an important cell surface antigen, to address the problem of current assay inaccuracy based on hydrolytic procedures and/or relatively non-specific and harsh methods. The third goal entails the implementation of a new automated color detection system for oligosaccharides that does not require prior destructive hydrolysis or chemical labeling with a chromophore. Currently no detection technique exists for neutral oligosaccharides that is effective for the visual detection of oligosaccharides beyond three repeat units.