Role and Regulation of Protein Phosphatase 5 in Brain

描述（由申请人提供）： 可逆性磷酸化是一种重要的和常见的调节机制， 大脑的许多功能，包括电兴奋和巩固 记忆。关于蛋白质的特性和调节， 激酶参与这些过程，但对这些过程知之甚少。 蛋白磷酸酶是信号转导激酶的专性伴侣， 转导我们的长期目标是了解 丝氨酸/苏氨酸蛋白磷酸酶5在中枢神经系统中的作用。我们最近 从脑中纯化了该酶作为脂质活化酶。虽然很少 已知蛋白磷酸酶5的生物学功能， 非神经元细胞在激素信号通路中涉及这种酶 控制基因表达、离子通道调节和其它过程， 是神经元功能的核心为了理解这一点 大脑中的酶，我们计划确定其在神经元中的细胞靶点， 内分泌细胞此外，我们将定义信号通路， 蛋白磷酸酶5参与大脑，以进一步了解如何 激素和神经递质控制神经元功能。

DESCRIPTION (provided by applicant): Reversible phosphorylation is an important and common mechanism for regulating many functions in brain including electrical excitation and the consolidation of memory. Much is known concerning the identity and regulation of protein kinases that participate in these processes, but far less is known about the protein phosphatases that are the obligate partners of kinases in signal transduction. Our long-term goal is to understand the function and regulation of Ser/Thr protein phosphatase 5 in the central nervous system. We recently purified this enzyme as a lipid-activated enzyme from brain. Although little is known about the biological function of protein phosphatase 5, studies in non-neuronal cells have implicated this enzyme in hormone signaling pathways controlling gene expression, ion channel regulation and other processes that are central to the function of neurons. In order to understand the role of this enzyme in brain, we plan to identify its cellular targets in neurons and endocrine cells. In addition, we will define signaling pathways in which protein phosphatase 5 participates in brain to further our understanding of how hormones and neurotransmitters control neuronal function.