Genomics resources and infrastructure for B. schlosseri

描述（由申请人提供）：本提案的目标是为殖民地原脊索动物Botryilus schlosseri创建基因组资源和基础设施。B. schlossei是一种实验室饲养的，基因可操纵的生物，其生活史包括许多独特的和实验可获得的发育和免疫过程，使其成为各种研究的潜在模式生物。其中包括一个类似于脊椎动物MHC控制的组织相容性系统，能够寄生在基因决定等级的相关个体上的干细胞，以及两个独立的发育程序——一个是有性的，另一个是无性的——这导致了形态和功能上相同的身体结构。原脊索动物代表了脊椎动物的祖先，这种密切的系统发育关系表明，控制这些生物现象的基因和调控机制与脊椎动物生物学有关。该提案将创建：一个完整的schlosseri基因组的遗传和初始物理图谱；规范化和排列的cDNA文库；从这些标准化文库中建立一个包含4- 5000个克隆的EST数据库，并开发转基因和/或瞬时基因表达系统。此外，这些资源将用于编制未来筹资所需的初步数据。

DESCRIPTION (provided by applicant): The objective of this proposal is to create the genomic resources and infrastructure for the colonial protochordate, Botryilus schlosseri. B. schlossei is a laboratory reared, genetically manipulable organism with a life history that includes a number of unique and experimentally accessible developmental and immunological processes, making it a potential model organism for a variety of studies. These include a histocompatibility system analogous to that controlled by the MHC in vertebrates, stem cells with the ability to parasitize related individuals in a genetically determined hierarchy, and two separate developmental programs- one sexual and the other asexual-that result in morphologically and functionally identical body plans. The protochordates represent the ancestral vertebrates, and this close phylogenetic relationship suggests that the genes and regulatory mechanisms that control these biological phenomena in Botryllus are Iconserved and relevant to vertebrate biology. This proposal will create: a complete genetic and initial physical map of the B. schlosseri genome; a number of normalized and arrayed cDNA libraries; an EST database of 4-5,000 clones from these normalized libraries, and the development of transgenic and/or transient gene expression systems. In addition, these resources will be used to generate preliminary data for future funding.