MOLECULAR DETERMINANTS OF DENGUE EPIDEMIC POTENTIAL

该项目旨在解决墨西哥登革热（DEN）的新问题。 登革病毒感染和登革出血热（DHF）已成为主要的公共卫生问题。 事实上，超过20亿人处于DEN感染的风险中，每年有数百万人感染，估计有10万例威胁生命的DHF病例，并且情况继续恶化。 不幸的是，导致DHF在20世纪60年代和70年代成为东南亚主要公共卫生问题的流行病学情况现在正在热带美洲和墨西哥发生。 病媒蚊子埃及伊蚊的流行率很高，另一个主要病媒白纹伊蚊正在入侵该国，可能使情况恶化。 多种血清型和菌株的登革病毒正在同时传播，而且危险的是，登革出血热的发病率正在迅速增加，登革病毒正在墨西哥北方的城市流行。在这项资助中，我们将与来自墨西哥城国家理工学院、蒙特雷新莱昂自治大学和梅里达尤卡坦自治大学的同事一起，调查和阐明DEN传播和毒力的载体和病毒遗传决定因素。这些信息将用于开发和表征对人类构成重大风险的载体和病毒的生物标志物。 这些信息可以用来集中有限的资源和人才，控制可能发生流行病的情况。 与DEN/DHF相关的载体和病毒基因型的定义将允许最终阐明决定病毒传播、疾病和流行潜力的机制，并可能导致北美和其他地方DEN的新控制和监测策略。 这些拟议的研究还将提供宝贵的信息，说明如何将已开发的新技术和方法应用于对当前和未来的公共卫生具有重要意义的其他病媒传播疾病。

This project addresses emerging problems with Dengue (DEN) in Mexico. DEN and dengue hemorrhagic fever (DHF) have emerged as major public health problems. Indeed, more than 2 billion people are at risk for DEN infection, millions are infected annually with an estimated 100,000 cases of life threatening DHF, and the situation continues to deteriorate. Unfortunately, the epidemiologic circumstances that led to the emergence of DHF as a major public health problem in Southeast Asia in the 1960s and 1970s are now occuring in tropical America and Mexico. The vector mosquito, Aedes aegypti, is hyperendemic, and another major vector, Aedes albopictus, is invading the country and may exacerbate the situation. Multiple serotypes and strains of DEN are now circulating simultaneously, and ominously, the incidence of DHF is increasing rapidly and DEN epidemics are occuring in cities in Northern Mexico. In this grant, we will, with colleagues from the Instituto Politecnica Nacional in Mexico City, from the Universidad Autonoma of Nuevo Leon in Monterrey, and the Universidad Autonoma of the Yucatan in Merida, investigate and elucidate the vector and viral genetic determinants of DEN transmission and virulence. This information will be used to develop and characterize biomarkers of vectors and viruses that pose substantial risk to human populations. Such information could be used to focus limited resources and and talents for control on situations with epidemic potential. Definition of vector and virus genotypes associated with DEN/DHF will permit eventual elucidation of mechanisms that determine virus transmission, disease, and epidemic potential and may lead to novel control and surveillance strategies for DEN in North America and elsewhere. These proposed studies will also provide invaluable information concerning the application of developed new technologies and approaches to other vector borne diseases of current and future public health importance.