CORE-- Neuroanatomy-Imaging

根据审查小组的要求，从神经解剖成像核心中删除了定量光学显微镜（LM）密度测定法的使用。此外，还作了一些修改，反映了项目提案的变化和项目4的删除。特别是，LM和EM放射自显影，DiA顺行追踪，TUNEL标记技术已被添加，和aptacellular标记，biocytin顺行追踪，LM重建技术已被删除。抗血清也有变化，详细描述：抗孕酮受体，α 2A-肾上腺素能受体， 受体和p22-phox，而针对5 HT受体、c-Fos、nNOS和加压素产生的抗血清已被删除。项目对神经解剖成像核心的利用率也发生了变化，反映了项目4的删除。经修订的核心说明如下。主要变化由左边空白处的一条线表示。总的来说，预算减少了。预算变动的详细说明已列入预算说明。

As requested by the review team, the use of quantitative light microscopic (LM) densitometry has been removed from the Neuroanatomy-lmaging Core. In addition, changes have been made that reflect changes in the project proposals and the deletion of Project 4. Specifically, LM and EM autoradiography, DiA anterograde tract-tracing, and TUNEL labeling techniques have been added, and juxtacellular labeling, biocytin anterograde tract-tracing, and LM reconstruction techniques have been deleted. There also have been changes in the antisera that are detailed: descriptions of antisera raised against the progesterone receptor, the alpha2A-adrenergic receptor and p22-phox are now included, while antisera raised against 5HT receptors, c-Fos, nNOS and vasopressin have been deleted. There have also been changes in the percent utilization of the Neuroanatomy-lmaging Core by the projects reflecting the deletion of Project 4. The revised description of the core is provided below. Major changes are indicated by a line in the left margin. Overall, the Budget has been reduced. A detailed account of the budgetary changes has been included in the Budget Justification.