Unbiased determination of BRAF variants modulating BRAF - CRAF dimerization and MAPK signalling

的博士学生将利用一系列实验方法，包括分子生物学技术和生物信息学，生成先进基因组编辑工具。这些工具将用于研究RAF激酶家族及其在细胞增殖和转化中信号传导的分子机制。为此，博士生将学习细胞生物学、病毒和非病毒递送方法、细胞分析、生物化学、下一代测序和多组学等先进技术，并有机会收集最新基因组编辑策略的知识。该项目是Babraham研究所Simon Cook实验室与阿斯利康Discovery Sciences合作的项目。的博士学生将首先在英国剑桥的阿斯利康的精确基因组编辑团队工作，然后在英国剑桥的Babraham研究所的信号实验室工作。

The Ph.D. student will generate advanced genome editing tools utilizing a range of experimental methods including molecular biology techniques and bio-informatics. These tools will be used to study the RAF kinase family and the molecular mechanisms of their signalling in cell proliferation and transformation. To this end, the PhD student will learn advanced technologies of cell biology, viral and non-viral delivery methods, cellular assays, biochemistry, next generation sequencing and multi-omics as well as an exciting opportunity to gather knowledge in the latest genome editing strategies.This project is a collaboration between Simon Cook's lab at the Babraham Institute and Discovery Sciences in AstraZeneca. The Ph.D. student will be based initially in the Precise Genome Editing team at AstraZeneca, in Cambridge UK, and successively in the Signalling Laboratory, at the Babraham Institute, in Cambridge UK.