RAMAN SPECTROSCOPY FOR CANCER DIAGNOSIS AND MONITORING

描述（来自申请人摘要的逐字）：我们的总体目标是 使振动光谱学能够应用于癌症诊断， 治疗监测。在癌症的最早阶段进行检测， 这是至关重要的，因为它大大提高了成功治疗的可能性。 传统的诊断方法依赖于物理切除一部分 组织和形态学的显微镜评估。需要切除组织 减少了可以取样的组织面积。一种非侵入性技术 消除这个问题。此外，一种非侵入性技术有可能 以允许治疗在用于治疗的相同内窥镜手术期间开始 诊断和减少与组织切除相关的其他并发症， 组织处理和增加患者感染的风险。我们将重点 关于发展拉曼光谱检测癌前条件， Barrett食管患者巴雷特食管是一种病理学， 鳞状上皮衬里被一种特殊的化生组织所取代 上皮和腺癌的可能性增加。因为 不典型增生的显微镜变化难以观察，整个区域的 应取样化生上皮。因此，巴雷特食管是 非常适合非侵入性诊断技术。的方法和技术 在该提议中开发的技术也可以应用于其它组织， 子宫颈我们工作的第二个目标是发展拉曼光谱， 评估治疗效果的方法。目前的监测方法 单个肿瘤对治疗反应是不可靠的，且通常 在治疗过程中难以实施。无创性的发展 或微创光学方法来可靠地识别细胞凋亡区域 和坏死将提供一种简单的方法来测定肿瘤反应， 个别癌症患者。因此，可以定制治疗。

DESCRIPTION (Verbatim from Applicant's Abstract): Our general objective is to enable the application of vibrational spectroscopy to cancer diagnosis and treatment monitoring. The detection of cancer at its earliest stages is crucial, for it greatly improves the likelihood of successful treatment. Traditional methods of diagnosis have relied on physical removal of a portion of tissue and microscopic assessment of morphology. The need for tissue removal reduces the area of tissue that can be sampled. A noninvasive technique would eliminate this problem. Furthermore, a noninvasive technique has the potential to allow treatment to begin during the same endoscopic procedure used for diagnosis and reduce other complications associated with tissue removal such as tissue handling and increased risk of infection to the patient. We will focus on developing Raman spectroscopy for detection of precancerous conditions in patients with Barrett's esophagus. Barrett's esophagus is a pathology in which the squamous epithelial lining is replaced by a specialized metaplastic epithelium and the likelihood of adenocarcinoma is increased. Because the microscopic changes of dysplasia are difficult to observe, the entire area of metaplastic epithelium should be sampled. Therefore, Barrett's esophagus is well-suited for a noninvasive diagnostic technique. The methods and techniques developed in this proposal may also find application in other tissues such as the cervix. The second goal of our work is to develop Raman spectroscopy as a method for assessing the effects of treatment. Current methods for monitoring the response of an individual tumor to therapy are unreliable and often difficult to implement during the course of therapy. Development of noninvasive or minimally-invasive optical methods to reliably identify regions of apoptosis and necrosis would provide a simple method for assaying tumor response in each individual cancer patient. Consequently, treatments could be customized.