Gene expression in regenerating adult vertebrate CNS.

描述（由申请方提供）：成年哺乳动物中枢神经系统（CNS）损伤通常是永久性和进行性的，可能是疾病或创伤性损伤的结果。许多最近的研究取得了有限的成功，可能是由于使用了最喜欢的模型不能中枢神经系统再生。为了进一步了解成年人的CNS再生，并将这些知识应用于临床水平，必须在没有外来干扰的情况下能够再生的成年人模型中阐明这些因素。了解这些因素是什么，以及它们如何驱动恢复，将有助于进一步了解整个神经再生，以及基于通过这些途径的靶向通量的治疗方案。该研究培训应用涉及成年非洲爪蟾，一种能够将颅神经再生到CNS中的脊椎动物模型，导致前庭听觉神经（nVIII）功能的恢复。具体目标是：（1）发现成年脊椎动物nVIII中驱动再生的基因;（2）功能的行为恢复的定量;和（3）nVIII损伤和再生程度的解剖学验证。将基因与正常发育中涉及的基因和不能CNS再生的模型进行比较。

DESCRIPTION (provided by applicant): Injury to the adult mammalian central nervous system (CNS) is usually permanent and progressive, and can be the result of disease or traumatic injury. Many recent studies have had limited success, possibly due to use of favorite models incapable of CNS regeneration. In order to further understand CNS regeneration in the adult, and to apply this knowledge on the clinical level, these factors must be elucidated in an adult model capable of regeneration in the absence of extraneous perturbations. Understanding what these factors are, and how they act to drive recovery, will allow further understanding of neural regeneration as a whole, and treatment options based on targeted flux through these pathways. This research training application involves adult Xenopus laevis, a vertebrate model capable of cranial nerve regeneration into the CNS, resulting in recovery of function of the vestibuloauditory nerve (nVIIl). The specific aims are: (1) discovery of genes driving regeneration in the adult vertebrate nVIII; (2) quantification of behavioral recovery of function; and (3) anatomical verification of nVIII lesion and extent of regeneration. Genes will be compared with those implicated in normal development and with models incapable of CNS regeneration.