CONTROL OF CELL SHAPE BY THE FRC2 GENE IN ARABIDOPSIS

18.研究金培训和职业目标研究金培训的最终目标是培养一名在遗传学和分子生物学方面接受多学科培训的全面研究人员，并提供发育生物学方面的广泛背景。为了实现这一目标，奥本海默博士和我制定了一项研究计划，将课程作业和实验室经验结合起来。本课程将为问题解决能力、抽象思维过程和创造力的发展提供框架。我们认为这些课程将帮助我发展成为一名具有广泛分析思维能力的研究人员。此外，拟议的研究金培训将提供关于经典遗传分析(单突变和双突变分离、分析和遗传图谱)和现代分子技术(克隆、双杂交分析、转基因植物构建)的正式培训。我们相信，通过参加发展这些技能的研究培训，同时融入遗传学和分子生物学中经常使用的技术，我将发展成为一名独立的研究员，其培训将成为研究界的一笔财富。19.赞助人姓名及学位(S)奥本海默，G.B.S.博士20.职位/职级助理教授研究兴趣/领域植物细胞形态的遗传和分子控制、细胞分裂控制、植物动蛋白功能、植物毛形态发生、植物发育研究建议22。描述(不超过所提供的空间)拟议研究的长期目标是更深入地了解细胞形成其形状的过程。虽然，植物拟南芥是这项研究计划的主题，但从了解植物细胞发育中获得的知识可能会应用于动物的细胞发育。这项提案中提到的基因之一Zwi就说明了这一点。Zwi的产品KCBP此前被认为仅限于植物界。最近，在动物细胞(海胆)中发现了一种相关的蛋白质，Kinesin-C，在那里它被认为在发育中发挥作用。因此，在植物中与KCBP相互作用的基因在动物细胞中可能有未被发现的对应物。因此，从对影响植物细胞形状的基因的研究中学到的东西可能在更广泛的范围内适用于人类。这项拟议研究的近期目标是鉴定FRC2基因产物，并测试其在细胞形态发育中的功能预测。FRC2基因产物已知控制拟南芥的毛状体分支数量。这项研究的具体目标是：克隆FRC2基因并鉴定其产物，直接检验FRC2对Zwi和FRC4都是冗余的假设，检验FRC2产物与Zwi产物相互作用的假说，并分离FRC突变的抑制子和/或修饰子。PHS416-1(Rev.12/98)表第2页bb抄送名称(姓氏、首字母、中间首字母)Conte Jeffries，StaceyJ页码的个人NRSA应用程序表(页码在整个申请过程中在底部连续编号。不要使用第1节申请者的后缀，如5a、5b。)首页(项目1-8、.15)、表单页面2(项目16-18、22)和目录========================================Section End===========================================

18. GOALS FOR FELLOWSHIP TRAINING AND CAREER The ultimate goal of this fellowship training is to create a well-rounded researcher with multidisciplinary training in Genetics and Molecular Biology, as well as provide a broad background in Developmental Biology. To achieve this goal, Dr. Oppenheimer and I have developed a research plan that will incorporate both course work and lab experience. The coursework will provide the framework for the development of problem solving abilities, abstract thinking processes, and creativity. We feel these courses will help me develop into a researcher with extensive analytical thinking abilities. In addition, the proposed fellowship training will provide formal training in classical genetic analysis (single and double mutant isolation, analysis and genetic mapping) and modern molecular techniques (cloning, two-hybrid analysis, construction of transgenic plants). We believe that by participating in research training that develops these skills, while incorporating techniques frequently used in Genetics and Molecular Biology, I will develop into an independent researcher whose training will be an asset to the research community. SPONSOR 19. NAME AND DEGREE(S) Oppenheimer, David G. B.S.Ph.D. 20. POSITION/RANK Assistant Professor 21. RESEARCH INTERESTS/AREAS Genetic and molecular control of plant cell shape, cytoskeltalcontrol of plant cell expansion, plant kinesin function, plant trichome morphogenesis, plant development RESEARCH PROPOSAL 22. DESCRIPTION (Do not exceed space provided) The long-term goal of the proposed research is to gain greater insight into the processes by which cells obtain their shape. Although, the plant Arabidopsis thaliana is the subject of this research proposal, it is possible that the knowledge gained from understandingcell development in plants can be applied to cell development in animals. This is illustrated by one of the genes mentioned in this proposal, ZWI. The ZWI product, KCBP, was previously thought to be confined to the plant kingdom. Recently, a related protein, Kinesin-C, has been identified in animal cells (sea urchin) where it is thought to play a role in development. Therefore, genes that interact with KCBP in plants may have undiscovered counterparts in animal cells. Thus, what is learned from studying the genes that contribute to plant cell shapes may be applicable on a broader scope to humans. The immediate goals of the proposed research are to identify the FRC2 gene product, which is known to control trichome branch number in Arabidopsis, and test the predictions about its function in cell shape development. The specific objectives of the proposed research are: To clone the FRC2 gene and identify its product, to directly test the hypothesis that FRC2 is redundant to both ZWI and FRC4, to test the hypothesis that the FRC2 product interacts with the ZWI product, and to isolate suppressors and/or modifiers of the frc mutation. PHS416-1 (Rev. 12/98) Form Page 2 BB cc NAME (Last, first, middle initial) Individual NRSA Appliq Table of Conte Jeffries, StaceyJ Page Numbers (Number pages consecutively at the bottom throughout the application. Do not use Section 1Applicant suffixes such as 5a,5b.) Face Page (Items 1 -8,.15), Form Page 2 (Items 16-18, 22), and Table of Content ========================================Section End===========================================