ANIMAL CORE

动物核心B的主要目标是继续提供SAMP1/YitFc(SAMP)小鼠，供构成计划项目的每个单独项目使用。动物核心将在统一的环境条件下，在一个共同的集中设施中维护SAMP繁育群体及其衍生群体，包括SAMP同源品系，以及亲本对照(AKR)群体。上述小鼠的饲养对该计划的成功至关重要，特别是考虑到有证据表明，环境因素，如特定共生细菌的定植，可以影响疾病的存在或病程。动物生产的集中化还通过消除多余的繁殖群体来降低成本，并最终通过确保实验动物的有效利用来减少实验所需的动物总数。 五个项目中的每个项目都提供了MICE。此外，动物核心对实验小鼠分布的协调使五个项目之间的沟通和合作达到最佳状态。第二个具体目标是继续为研究人员提供SAMP小鼠肠道炎症和其他形态参数的集中组织学分析，以监测随着时间的推移表型和组织病理学的变化。动物核心的这一部分的一个主要优势是继续可获得经验丰富的Gl病理学家(CA Moskaluk)对SAMP组织进行定期组织分析，并就该模型中疾病的病理特征向PPG研究人员提供持续的咨询服务。第三个具体目标是继续作为一个资源，为小鼠提供在SAMP背景株上特别引入的基因突变，这将有助于 该计划内有多个项目。动物核心的这一部分与弗吉尼亚大学的小鼠遗传学核心设施密切互动，由Marcia McDuffie博士(项目2的首席研究员)指导。使用SPEED同源基因技术，可以在三到四代内将这些诱导突变转移到合适的背景中。Theresa Pizarro博士将继续指导计划项目Animal Core的总体活动，一个由核心主任、核心顾问(CA Moskaluk)和计划项目PI(F Cominelli)组成的委员会将定期开会，解决常规质量控制和优先事项问题。此外，由咨询委员会成员组成的监督委员会将每年审查核心的整体运作情况。

The primary aim of the Animal Core B is to continue to provide SAMP1/YitFc (SAMP) mice for use in each of the individual projects comprising the Program Project. The Animal Core will maintain the SAMP breeding colony, its derivations including SAMP congenic lines, as well as the parental control (AKR) colony in a common centralized facility under uniform environmental conditions. Housing of the aforementioned mice is critical to the success of the program, particularly in the light of evidence that environmental factors, such as colonization of specific commensal bacteria, can affect the presence or course of disease. Centralization of animal production also reduces costs, by eliminating redundant breeding colonies, and ultimately reduces the overall number of animals required for experimentation by ensuring efficient utilization of experimental mice by each of the five projects. In addition, coordination of the distribution of experimental mice by the Animal Core allows optimal communication and collaboration between each of the five projects. A second specific aim is to continue to provide investigators with centralized histological analyses of intestinal inflammation and other morphological parameters in SAMP mice, to monitor phenotypic and histopathological changes over time. A major strength of this component of the Animal Core is the continued availability of an experienced Gl pathologist (CA Moskaluk) to perform periodic histological analyses of SAMP tissues and to provide ongoing consultative services to PPG investigators regarding the pathological features of disease in this model. The third specific aim is to continue to serve as a resource for providing mice with specifically introduced genetic mutations onto the SAMP background strain that will be useful to multiple projects within the program. This component of the Animal Core interacts closely with the Mouse Genetics Core Facility at UVA, directed by Dr. Marcia McDuffie (Principal Investigator of Project 2). Using speed congenic technology, transfer of these induced mutations into the appropriate background can be accomplished in three to four generations. Dr. Theresa Pizarro will continue to direct the overall activities of the Program Project Animal Core, and a committee consisting of the Core Director, Core Consultant (CA Moskaluk), and the PI of the Program Project (F Cominelli), will meet on a regular basis to address routine quality control and prioritization issues. In addition, an oversight committee consisting of members of the Advisory Board will review the overall operation of the Core on a yearly basis.