CORE--RESEARCH

研究核心实验室，核心B，由胡安·卡帕索博士指导，是科罗拉多大学健康科学中心肾脏疾病和高血压部门的一个资源设施。它由实验室组成，作为方案项目所有调查人员的核心设施。研究核心提供必要的通用设备和用品，由方案项目的所有调查人员共享。具体地说，核心B包括一个组织培养设施/实验室(Milli-Q水系统、二氧化碳孵化器、层流罩、显微镜、冷冻库、用品/试剂)、大型 其中包括一间设备间(分光计、冰箱、冰柜、酸碱计、离心机、胶片显影剂、聚合酶链式反应、分析仪及电泳仪)、一间放射性实验室(贝塔及伽马计数器及放射性废物处置容器)、一间洗碗室(烘干炉、高压灭菌器等)和一间动物外科实验室(解剖显微镜、外科仪器及用品、秤、泵、代谢笼、束缚装置、孵化器、小型及微量红细胞压积离心机、加热手术台、小型动物血流计/肾脏血流和血压系统、尾袖血压分析仪)。此外，还将保存一份标准化学品/试剂、玻璃器皿和消耗性用品的库存以供使用 所有的调查人员。这一核心还将支持这些设施的服务合同和维修。

The research core laboratory, Core B, directed by Dr. Juan Capasso, is a resource facility located within the Division of Renal Diseases and Hypertension at the University of Colorado Health Sciences Center. it is comprised of laboratories set up as a core facility for all the investigators of the program project. The research core provides access to the necessary general equipment and supplies shared by all of the investigators of the program project. Specifically, the Core B includes a tissue culture facility/laboratory (Milli-Q water system, CO2 incubators, laminar flow hoods, microscopes, cryostorage, supplies/reagents), large equipment rooms (spectrophotometers, refrigerators, freezers, pH meters, centrifuges, film developer, PCR, analyzers and electrophoresis equipment), a radioactivity laboratory (beta and gamma counters and radioactive waste disposal containers), a dishwashing room (drying oven, autoclave etc), and an animal surgery laboratory (dissecting scopes, surgical instruments and supplies, scales, pumps, metabolic cages, restraining devices, incubator, small and micro hematocrit centrifuges, heated surgical tables, small animal blood flow meter/renal blood flow and blood pressure system, tail cuff blood pressure analyzer). In addition, an inventory of standard chemicals/reagents, glassware and expendable supplies will be maintained for use by all the investigators. This core will also support the service contracts and repair of these facilities.