Development of an Acoustic Biological Shaker

描述（由申请人提供）：这个小企业创新研究第一阶段项目将证明使用ResonantAcoustics（低频声混合）作为开发高效实验室规模ResonantAcoustic生物振荡器（RBS）的基础的可行性。传统的生物轨道振荡器具有很大的缺点，包括氧气转移能力低以及扩大到搅拌罐生物反应器时适用性有限。相比之下，RBS将提供上级传质（增加的氧传递和减少的混合时间）。在项目实施过程中，将设计一种新型封闭装置，以充分利用增强的声混合。RBS的操作特性必须优化以最大化生物培养物的生长和产物表达。RBS的性能将与用于细菌、真菌和动物细胞培养的轨道振荡器进行比较。预计RBS将能够支持显著增加的生物质水平，由于大大增强了氧气传输。RBS相对于常规技术的优势随着培养物粘度的升高而增加，这对于真菌培养物将是特别有利的。一个非常上级的实验室振荡器的可用性将在增加生物技术实验室开发新药的工艺开发速度方面产生重大影响。

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I project will demonstrate the feasibility of using ResonantAcoustics (low-frequency acoustic mixing) as the basis for developing a highly efficient laboratory scale ResonantAcoustic Biological Shaker (RBS). Conventional biological orbital shakers have substantial drawbacks that include low oxygen transfer capability and limited applicability when scaling up to stirred-tank bioreactors. The RBS, in contrast, will provide superior mass transfer (increased oxygen transfer and reduced mixing times). During the course of project, a new type of closure will be designed in order to take full advantage of the enhanced acoustic mixing. Operating characteristics of the RBS must be optimized to maximize the growth and product expression of biological cultures. Performance of the RBS will be compared to orbital shakers for bacterial, fungal, and animal cell cultures. It is anticipated that the RBS will be able to support dramatically increased biomass levels over orbital shakers due to substantially enhanced oxygen transfer. The advantage of the RBS over conventional technology increases as culture viscosity rises, which will be especially advantageous for fungal cultures. The availability of a substantially superior laboratory shaker will have a substantial impact in increasing the pace of process development for biotechnology labs developing new Pharmaceuticals.