Analysis of Gene Expression in Thoracic Malignancies

荧光蝶啶为基础的核苷类似物，可以通过磷酸二酯键位点特异性掺入DNA寡核苷酸已开发和专利。一系列的这些化合物的荧光性能进行了表征和潜在的应用正在调查中。这些新的荧光团是研究蛋白质/DNA相互作用的有前途的探针，因为它们不与DNA外部连接，并且它们似乎不会破坏DNA的三级结构。已经开发了用于HIV-1整合酶和烷基鸟嘌呤-DNA-烷基转移酶的基于荧光的快速测定，并且这些荧光团作为杂交探针的效用已经获得专利并且正在调查中。一些调查这些新研究工具的潜在应用的合作研究也在进行中，这些探针已获得Trilink Technologies的许可，并已上市。

Fluorescent pteridine-based nucleoside analogs that can be site-specifically incorporated into DNA oligonucleotides through a phosphodiester linkage have been developed and patented. The fluorescence properties of a series of these compounds have been characterized and potential applications are being investigated. These new fluorophores are promising probes for the study of protein/DNA interactions, because they are not attached to DNA externally and they do not appear to disrupt the tertiary structure of DNA. Rapid fluorescence-based assays for HIV-1 integrase and alkylguanine-DNA-akyltransferase have been developed, and the utility of these fluorophores as hybridization probes has been patented and is under investigation. A number of collaborative studies investigating the potential applications of these new research tools are also underway, and the probes have been licensed by Trilink Technologies and are commercially available.