CORE--MICROSCOPY AND IMAGING CENTER

细胞生物学核心的主要目标是促进定量定性成像技术的使用，并在细胞和分子水平上分析运输过程。适用于细胞、组织、整个器官以及野生型、转基因和突变动物研究的成像技术的范围已经大大扩大。新技术现在可用于特定目标蛋白的更高分辨率定位，以及允许动态变化可以识别的重要显微镜。这些包括免疫细胞化学、共聚焦显微镜、电子显微镜、原位杂交、无偏立体形态测定和生命显微镜。这些技术在细胞生物学和生理学中非常有用，但对于没有先前培训或经验的研究人员来说并不容易获得。此外，选择适当的技术或技术组合需要口译经验和对技术局限性的了解

The major goal of the Cell Biology Core is to facilitate the use of quantitative qualitative imaging techniques and in the analysis of transport processes at the cellular and molecular level. The range of imaging techniques which are applicable to the study of cells, tissues, whole organs and in wild type, transgenic and mutant animals have been greatly expanded. New technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. These include immunocytochemistry, confocal microscopy, electron microscopy, in situ hybridization, uunbiased stereological morphometry, and vital microscopy. These techniques are extremely useful in cell biology and physiology but are not easily accessible to investigators without previous training or experience. In addition, the selection of the proper technique or combination of techniques requires experience in interpetation and knowledge of the limitations of the method. The core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, and electron microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the core will provide the facilities, service and education necessary for the efficient application of imaging techniques tailored to the individual research objectives of the various members.