VIRAL IMMUNOLOGY CORE

CORE E描述（由申请人提供）：该提案描述了NYU CFAR病毒免疫学核心将提供的设施和服务。本核心的主要目的是：a)提供一系列检测方法，以测量参与HIV免疫防御的各种细胞的功能和特征，受HIV感染的细胞的功能，以及细胞的HLA类型（当需要进行各种细胞检测时）；b)测试多克隆血清以及人类、灵长类动物和啮齿动物抗HIV单克隆抗体（mab）的抗HIV活性、对多种HIV初级分离物的中和活性，以及它们与完整病毒粒子和受感染细胞结合的能力；c)病毒分离和鉴定，以便这些病毒可用于上述免疫分析；d)构建和生产用于上述检测的假病毒；e)向CFAR研究者和艾滋病患者提供人类抗hiv抗体

CORE E DESCRIPTION (provided by applicant): This proposal describes the facilities and services to be provided by the Viral Immunology Core of the NYU CFAR. The primary purpose of this Core is a) Provision of a range of assays to measure the function and characteristics of various cells involved in immune defense against HIV, the function of cells affected by HIV infection, and, the HLA type of cells (when this information is needed to perform various cellular assays); b) Testing of polyclonal sera as well as human, primate and rodent anti-HIV monoclonal antibodies (mAbs) for anti-HIV activity, for neutralizing activity against a wide range of HIV primary isolates, and for their ability to bind to intact virions and to infected cells; c) Virus isolation and characterization so that these viruses can be used in the aforementioned immunologic assays; d) Construction and production of pseudoviruses for use in the aforementioned assays; e) Provision of human anti-HIV mAbs to CFAR investigators and AIDS researchers around the world; f) Sequencing of Ig and T cell receptor genes for studies of gene usage in immune responses in the setting of HIV infection; and g) Provision of training to investigators, students, postdocs, international fellows, Infectious Disease fellows, and technicians, within and outside of CFAR, in the techniques enumerated above. The services of this core are available to the NYU AIDS research community, to NYU researchers in other fields (such as members of the NYU Cancer Center), to NIH supported researchers outside of NYU, and to those scientists around the world who need mAbs for meritorious HIV-related projects. A charge-back system is in place and contributes to the support of the services provided.