Coordination of localized actin polymerization with endosomal membrane dynamics

为了让细胞对环境做出反应，它们必须利用几种途径将分子移动到细胞内正确的目的地，在那里信号可以被处理，它们的行为可以进行反应调整。一旦分子被内化，它们就会被分拣到不同的路径中，而不是分拣隔间。然而，细胞如何能够识别分子并将其分类到正确的途径仍不清楚。最近的证据表明，WASH产生的肌动蛋白网络与这些分类隔间的功能有关。使用低温相关成像方法，我们将能够以一个新的视角观察这个系统，以深入了解WASH及其肌动蛋白网络在这个分选过程中的参与。这将提高我们对细胞信号传递以及细胞如何适应环境提示的理解，这将是至关重要的，因为信号传递过程中的错误是导致许多病理情况的原因。此外，我们的工作包括开发优化的低温相关成像设备和评估新兴成像技术的程序。

For cells to respond to their environment, they must employ several pathways to move molecules to the correct destination within the cell where signals can be processed, and their behaviour can be adjusted in response. Once molecules are internalized, they are sorted into different pathways from sorting compartments. However, how the cell is capable of recognizing and sorting molecules into the correct pathways remains unclear. Recent evidence implicates the WASH generated actin network in the function of these sorting compartments. Using a cryogenic correlative imaging approach, we will be able to observe this system with a novel perspective to gain insight into the involvement of WASH and its actin network in this sorting process. This will improve our understanding of cell signalling and how cells adapt in response to environmental cues, which will be essential as errors in signalling processes are responsible for numerous pathological conditions. Additionally, our work includes a component of development of optimized cryogenic correlative imaging equipment and procedures for the evaluation of emerging imaging technology.