Acquisition of an electrospray TOF mass spectrometer

说明（由申请者提供）：申请资金用于购置一台带有电喷雾电离源（Q-Tof）的飞行时间质谱仪。对拟议用户的研究方案至关重要的所需仪器的独特功能是：（高达32，000 u）能够检测和表征大的完整大分子物质;（ii）串联能力，允许产生质量选择的多肽离子的结构诊断片段;（iii）接近100%的占空比，允许肽和蛋白质离子的灵敏检测以及在扩展的质量范围内的短采集时间。所要求的仪器将用于研究辅助折叠和易位的机制;转铁蛋白家族及其受体的铁转运蛋白的金属结合特性和动力学;超氧化物歧化酶家族的新型氧化还原活性蛋白的结构和动力学;以及膜信号转导和趋化性机制。此外，所要求的质谱仪将用于研究蛋白质成熟和降解的机制; RNA聚合酶的机制;以及核糖体结构和动力学。总共有7名教师（其中6名目前由NIH资助）将使用所要求的仪器。所要求的仪器将成为质谱中心的一部分，该中心由三个学术部门共同支持。中心主任将负责仪器的日常操作和维护。仪器的操作和保修期后的维护费用将通过向用户收费来收回，这一机制目前用于支持中心的其他四台质谱仪。该仪器的操作将由质谱咨询委员会监督，该委员会由代表麻省大学三个学术部门和一个跨学科研究中心的教职员工组成。

DESCRIPTION (provided by applicant): Funds are requested for acquisition of a time-of-flight mass spectrometer with an electrospray ionization source (Q-Tof). The unique features of the requested instrumentation that are critical for the research programs of the proposed users are: (i) high m/z range (up to 32,000 u) enabling detection and characterization of large intact macromolecular species; (ii) tandem capability allowing structurally diagnostic fragments of mass-selected polypeptide ions to be produced; (iii) duty cycle close to 100% allowing sensitive detection of peptide and protein ions and short acquisition times over an extended mass range. The requested instrumentation will be used to study mechanisms of assisted folding and translocation; metal binding properties and dynamics of iron transport proteins from the transferrin family and their receptors; structure and dynamics of novel redox-active proteins from superoxide dismutase family; and mechanisms of membrane signal transduction and chemotaxis. In addition, the requested mass spectrometer will be used for studies of mechanisms of protein maturation and degradation; mechanisms of RNA polymerases; as well as ribosomal architecture and dynamics. Altogether, 7 faculty members (6 of which are currently NIH funded) will use the requested instrumentation. The requested instrumentation will become a part of the Mass Spectrometry Center, which is jointly supported by three academic departments. The Director of the Center will be responsible for the daily operation and maintenance of the instrument. The cost of the instrument operation and upkeep beyond the warranty period will be recovered through users fees, a mechanism which is currently used for support of the other four mass spectrometers in the Center. The operation of the instrument will be overseen by a Mass Spectrometry Advisory committee comprised of faculty members representing three academic departments and one interdisciplinary research center at UMass.