Affymetrix Gene Chip Node System 3000

描述（由申请人提供）：要求为俄克拉荷马州医学研究基金会（OMRF）的中心设备设施提供Affymetrix(r)基因芯片/ r节点系统。所要求的仪器将为研究人员提供通过Affymetrix技术进行基因表达实验、大型重测序项目和人类基因组扫描单核苷酸多态性的高质量标准。此外，众所周知的Affymetrix技术提供了以低成本和非常高的可靠性非常快速地重新测序多达30,000个DNA碱基的变异的能力。最后，Affymetrix开发的阵列允许在含有单核苷酸多态性（snp）的大量基因组位点上测定等位基因。现在每个阵列的能力大约为10,000个snp，并计划在2004年达到1,000,000个snp，届时该仪器将首次提供给我们。OMRF和俄克拉何马大学的主要研究用户群体将使用该仪器及其配套的Affymetrix技术至少85%的容量进行基因表达、重测序和基因分型。这种能力将对目前正在进行的许多nih资助的项目产生实质性的影响，特别是通过提供现在无法获得的技术能力。这两种基因阵列系统现在在这个校园里可用，支持许多研究人员用更便宜和更不可靠的斑点阵列来收集基因表达阵列数据。这些设施都没有可比的基因分型或重测序或SNP基因分型能力。此外，我们怀疑所要求的设备的主要应用之一将是确认和扩展由其他更便宜的技术获得的基因表达结果。所要求的成套设备包括一个阵列读取器、一个计算机工作站、一个流体站和一个杂交站、多次培训课程、易于获得技术援助和第一年的维护。今后几年仪器使用支助和维修合同的费用将由OMRF承担。

DESCRIPTION (provided by applicant): An Affymetrix(r) Gene Chip/R Node System is requested for the central equipment facilities of the Oklahoma Medical Research Foundation (OMRF). The requested instruments will provide investigators with access to the high standard of quality available though the Affymetrix technology for gene expression experiments, large resequencing projects, and human genome scan single nucleotide polymorphisms. In addition, the well-known Affymetrix technology provides the capacity to very rapidly re-sequence up to 30,000 bases of DNA for variants at low cost and at very high reliability. Finally, Affymetrix has developed arrays that permit the determination of alleles at a large number of genomic sites containing single nucleotide polymorphisms (SNPs). The capability now is at approximately 10,000 SNPs per array and is planned to be >100,000 SNPs in 2004, when this instrument would first be available to us. Major investigator user groups at the OMRF and at the University of Oklahoma will use at least 85% of the capacity of this instrument and its accompanying Affymetrix technology for gene expression, for re-sequencing, and for genotyping. This capability will have a substantial impact upon a number of NIH-funded projects now underway, especially by providing technical capacity not now available to them. The two gene array systems now available on this campus support a number of investigators with less expensive and less reliable spotted arrays for gene expression array data collection. Neither of these facilities has comparable genotyping or re-sequencing or SNP genotyping capabilities. Moreover, we suspect that one of the major applications of the requested equipment will be to confirm and extend gene expression results obtained by other less expensive technologies. The equipment package requested includes an array reader, a computer workstation, a fluidics station, and a hybridization station, multiple training sessions, easy access to technical assistance, and maintenance for the first year. The expense for support for instrument use and the maintenance contracts in future years will be borne by the OMRF.