Gene Expression Array Scanner & Support Instrumentation

描述(由申请人提供)：本提案寻求资金用于购买仪器，使凯斯西储癌症中心基因表达阵列核心设施[GEACF]能够为其研究社区保持最先进的服务，同时满足快速增长的高样本数吞吐量和数据减少的需求。为了实现这一目标，这项建议要求为现有的CASE西部储备大学[CWRU]核心设施增加一个仪器包，并将提供以下内容：a)新的Affymetrix基因芯片微阵列扫描仪系统，以便能够以实验方式获得新一代基因表达微阵列；b)改进样本质量评估的系统，以促进转译研究样本的使用；c)最先进的数据管理包，旨在满足中高通量实验室的数据管理需求。 使用微阵列评估基因表达的能力是现代生物医学研究的重要资源。CWRU和克利夫兰大学医院的癌症中心于2000年1月建立了GEACF，以使他们的科学研究人员可以使用这项技术。基于Affymetrix技术的GEACF提供全方位服务，为基因表达技术的各个方面提供技术援助和建议。在过去的3年里，GEACF从一个为满足癌症中心研究人员的需求而建立的核心设施迅速发展成为一个为CWRU、其医学院及其附属机构[UHHS、MetroHealth和V.A.医院]以及克利夫兰临床基金会的研究人员处理样本的设施。GEACF通过处理来自组织培养、动物和人类的样本来支持不同的研究。目前强调的物种包括酵母、小鼠、大鼠和人。核心基金目前支持79名首席调查员的研究工作。然而，在接下来的两年里，我们预计我们的用户基础将显著扩大。在这方面，CWRU医学院将在来年完成一个大型建筑项目，该项目将带来超过22.2万平方米的在线面积。英国《金融时报》额外的实验室空间。GEACF设施将在为招募到这个新设施的研究人员和使用其动物资源中心的调查人员提供研究基础设施方面发挥重要作用，该中心将容纳70,000多只小鼠。

DESCRIPTION (provided by applicant): This proposal seeks funds to purchase instrumentation that will allow the Case Western Reserve Cancer Center Gene Expression Array Core Facility [GEACF] to maintain state-of-the art services for its research community, while simultaneously addressing rapidly increasing demands for high sample number throughput and data reduction. To accomplish this aim, this proposal requests funds for an instrument package to be added to the existing Case Western Reserve University [CWRU] Core Facility and will provide the following: a) A new Affymetrix GeneChip Microarray Scanner System to allow experimental access to the new generation of gene expression microarrays; b) A system to improve the assessment of sample quality to facilitate the utilization of samples from translational studies and c) A state-of-the-art data management package tailored to address the data management needs of moderate to high-throughput laboratories. The ability to assess gene expression using microarrays is an essential resource in modern biomedical research. The Cancer Center at CWRU and University Hospitals of Cleveland [UHC] established the GEACF in January 2000, to make this technology available to their scientific investigators. The full service Affymetrix technology-based GEACF offers technical assistance and advice on all aspects of gene-expression technology. Over the past 3 years, the GEACF has rapidly grown from a Core Facility established to address the needs of Cancer Center investigators to a Facility that processes samples for CWRU, its Medical School and its affiliated institutions [UHHS, MetroHealth and The V.A. Hospital] as well as for the research faculty of the Cleveland Clinic Foundation. The GEACF supports diverse research by processing samples harvested from tissue culture, animals and people. Species currently emphasized include yeast, mouse, rat and human. The Core Facility currently supports the research efforts of 79 principal investigators. However, over the next 2 years we anticipate a significant expansion of our User base. In this regard, the CWRU Medical School is completing a large building project over the coming year, which will bring on-line more than 222,000 sq. ft. of additional laboratory space. The GEACF Facility will play a significant role in providing research infrastructure for researchers recruited to this new facility and investigators using its animal resources center that will house more than 70,000 mice.