Acquisition of an ESI/APCI TOF Mass Spectrometer

描述（由申请人提供）：斯克里普斯研究所质谱中心正在寻求资金，用于购买电喷雾电离（ESI）和大气压化学电离（APCI）飞行时间（TOF）质谱仪，用于高精度MS和串联质谱实验。现有的MALDI FTMS仪器是Scripps七年前购买的，已经进行了超过25，000次分析，并被800多名研究人员使用。该中心急需这种高精度的ESI和APCI能力为其NIH资助的研究人员。现有的MALDI FTMS是首批设计之一，在分析不同类型的化合物方面具有非常有限的灵敏度和通用性。此外，MALDI FTMS的可靠性很差，因为每天的信号变化很大，而且真空系统在过去两年中已经重建了两次。该中心于1993年获得NIH资助，用于SCIEX API-3 ESI三重四极杆质谱仪，该质谱仪仍用于常规ESI-MS和ESI-MS/MS分析。然而，Scripps的这种仪器和所有其他仪器都不能提供ESI/APCI TOF反射器所能提供的高精度。ESI/APCI TOF反射器将有助于分析列出的研究人员和整个研究所的其他实验室所需的天然产物、代谢物、碳水化合物、肽、蛋白质、寡核苷酸和许多合成有机化合物。七个教师（如下所列）的研究将大大受益于ESI/APCI TOF反射器的功能。其中六名研究人员已经建立了NIH基金，第七名研究员菲利普·巴兰刚刚开始在斯克里普斯工作。仅这些群体就包括200多名博士后和研究生，还提供了一个包含8名小用户（均由NIH资助）的表格。与中心内的所有设备一样，ESI/APCI TOF反射器的使用也将从这一用户群扩展到整个研究所。

DESCRIPTION (provided by applicant): The Center for Mass Spectrometry at The Scripps Research Institute is seeking funds for the purchase of an electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) time-of-flight (TOF) mass spectrometer for high accuracy MS and tandem mass spectrometry experiments. The existing MALDI FTMS instrument was purchased by Scripps seven years ago and has performed over 25,000 analyses and is used by over 800 researchers. The Center is in critical need of this high accuracy ESI and APCI capability for its NIH funded researchers. The existing MALDI FTMS is one of the first designs and has very limited sensitivity and versatility with respect to analyzing different types of compounds. In addition, the MALDI FTMS reliability is poor as signal variability from day to day is high and the vacuum system has been rebuilt twice in the last two years. The Center received NIH funding in 1993 for a SCIEX API-3 ESI triple quadrupole mass spectrometer, which is still being used for routine ESI-MS and ESI-MS/MS analyses. However this and all other instrumentation at Scripps does not offer the high accuracy that the ESI/APCI TOF-reflectron would offer. The ESI/APCI TOF reflectron would facilitate the analysis of natural products, metabolites, carbohydrates, peptides, proteins, oligonucleotides and a host of synthetic organic compounds required by the researchers listed and other laboratories throughout the institute. The research of seven faculty (listed below) would greatly benefit from ESI/APCI TOF reflectron capabilities. Six of these researchers have established NIH funding, the seventh investigator, Philip Baran, has just begun at Scripps. These groups, alone, include over 200 postdoctoral associates and graduate students, a table of eight minor users (all NIH funded) is also provided. As with all the equipment within the Center, the use of the ESI/APCI TOF reflectron would also extend beyond this user group to the entire institute.