Core B--Immunological Monitoring

免疫监测核心B是SCCOR赠款中非常重要的组成部分。该核心将协助所有四个科学项目，并将向其他核心提供必要的信息。这一核心的具体目标是： 目的1：获取、分类和储存外周血单核细胞、血浆和血清，以及 心内膜心肌活检组织标本。 目的2：在临床和基础项目中，按照个体研究人员的要求对标本进行处理，以进行实验方案。 目的：建立每个患者外周血中的人淋巴母细胞样细胞系(LCL)，并在体外研究中维持EBV细胞系。 目的：建立、评价和实施免疫效应细胞的体外监测方法，以确定免疫效应细胞的频率和活性，为基础和临床项目提供支持。 该核心将向研究人员提供从参与各种研究的儿科心脏和心肺移植接受者那里获得的标本。为了评估生物疗法的免疫调节效果和/或EBV负荷增加的影响，这些系列样本将在Core开发和验证的一系列分析中进行测试。这些检测将识别、量化和表征循环中的同种反应性T细胞。其他检测方法将测量和表征这些患者外周血中EBV特异性T细胞的免疫缺陷。这类检测方法的发展及其在治疗过程中评估免疫功能的应用是核心的重要组成部分。核心意志 同时执行项目1-3所需的人类白细胞抗原DNA分型。

The Immunological Monitoring Core B is a very essential component of the SCCOR grant. This core will assist all four scientific projects and will provide the necessary information to the other cores. The Specific Aims of this core are: Aim 1: To procure, triage and store peripheral blood mononuclear cells, plasma and serum, and endomyocardial biopsy tissue specimens. Aim 2: To process the specimens as required by individual investigators for experimental protocols in the clinical and basic projects. Aim 3: To establish the human lymphoblastoid cell lines (LCL) from peripheral blood for each patient, and maintain the cell lines for EBV in vitro studies. Aim 4: To develop, evaluate and perform in vitro monitoring assays to determine the frequency and activity of immune effector cells in order to support basic and clinical projects. The core will make available to the investigators the specimens obtained from pediatric heart and heart-lung transplant recipients participating in the various studies. In order to assess the immune modulation effect of biotherapies and/or impact of elevation of EBV load, these serial specimens will be tested in a panel of assays developed and validated by the Core. These assays will identify, quantitate and characterize the allo-reactive T-cells in the circulation. Other assays will measure and characterize the immune defects of EBV-specific T cells in peripheral blood of these patients. The development of such assays and their application to assess of immune functions in the course of therapy are essential components of the Core. The Core will also perform HLA DNA typing required for projects 1-3.