CORE--NEUROPATHOLOGY CORE

神经病理学核心（NP Core）的主要目的是对爱因斯坦衰老研究（EAS）参与者的大脑进行诊断性神经病理学评估。为了实现这一目标，NP Core将其活动与Einstein/Montefiore病理学部门的随叫随到服务相结合，以便及时并根据协议采集EAS中登记的受试者的大脑。NP Core使用标准化的解剖和处理 使用以下方法进行程序并收集来自所有脑的神经病理学数据：a.大体检查和数码摄影，B。组织病理学评估，C。用硫代黄素-S荧光显微镜计数病变，d. PHF-tau免疫染色切片用于阿尔茨海默型病理学分期。对于血管病变的病例，对大体病变拍照，并记录病变的数量、类型和分布。在黑质神经元缺失或路易体的情况下，多个切片用突触核蛋白抗体免疫染色。筛选所有AD病例的杏仁核中的突触核蛋白免疫反应性路易体和4-重复tau免疫反应性嗜银颗粒。当检测到路易体时，路易体的密度在多个皮层区域和杏仁核中被确定。非阿尔茨海默病退行性痴呆的特征在于免疫细胞化学和抗体的神经丝，泛素和PHF-tau。小胶质细胞免疫组织化学也被常规用于证明不同的病理。NP Core为项目3提供固定和冷冻的大脑样本， 免疫细胞化学、图像分析和酶联免疫吸附测定。经管理核心批准，将组织提供给其他合格的研究者。NP Core确定载脂蛋白E基因型和TAU单倍型，并储存来自脑组织的DNA和RNA。还用pH和Western印迹法对HSP 70评估组织质量。将组织样本、载玻片和石蜡块储存起来，以备将来研究。报告提供给 使用标准化协议和程序将行政核心和数据提交给统计核心。

The major purpose of the Neuropathology Core (NP Core) is to perform diagnostic neuropathologic evaluations on brains of participants in the Einstein Aging Study (EAS). To accomplish this aim, the NP Core integrates its activities with the Einstein/Montefiore Pathology Departmental on-call service for harvesting brains of subjects enrolled in the EAS in a timely fashion and according to protocol. The NP Core uses standardized dissection and processing procedures and collects neuropathologic data from all brains with the following methods: a. Gross examination with digital photography, b. Assessment of histopathology, c. Lesion counts with thioflavin-S fluorescent microscopy, and d. PHF-tau immunostained sections for staging Alzheimer type pathology. For cases with vascular lesions, gross lesions are photographed and the number, type and distribution of lesions are documented. In cases with either neuronal loss or Lewy bodies in the substantia nigra, multiple sections are immunostained with antibodies to synuclein. The amygdala of all AD cases is screened for synuclein immunoreactive Lewy bodies and for 4-repeat tau immunoreactive argyrophilic grains. When detected, the density of Lewy bodies is determined in multiple cortical areas and the amygdala. Non-Alzheimer degenerative dementias are characterized with immunocytochemistry and antibodies to neurofilament, ubiquitin and PHF-tau. Microglial immunohistochemistry is also routinely used to demonstrate diverse pathologies. The NP Core provides fixed and frozen brain samples to Project 3 for immunocytochemical, image analytical and enzyme linked immunosorbent assays. Tissue is provided to other qualified investigators with approval of the Administrative Core. The NP Core determines apolipoprotein-E genotypes and TAU haplotypes and banks DNA and RNA from brain tissue. Tissue quality is also assessed with pH and western blots for HSP70. Tissue samples, slides and paraffin blocks are banked for future studies. Reports are provided to the Administrative Core and data to the Statistical Core using standardized protocols and procedures.