Protein Folding in the Cell

描述(由申请者提供)：我们申请资助2006年美国实验生物学家联合会(FASE B)关于“细胞中的蛋白质折叠”的夏季会议，将于2006年7月29日至8月3日在佛蒙特州萨克斯顿河举行。拟议的会议将是关于这一主题的一系列非常成功的FASEB会议中的第九次会议。这次会议的独特之处在于，它的目标是将细胞生物学家/遗传学家、生物物理学家/生物化学家和数学建模人员聚集在一起，以解决蛋白质折叠和错误折叠的复杂问题以及对人类疾病的影响。这是1990年第一次会议背后的概念，对于来自非常不同背景的科学家来说，这仍然是一个独特的机会。大多数蛋白质折叠会议要么考虑折叠的理论和体外方面，要么考虑分子伴侣和蛋白质折叠催化剂在细胞中的作用。然而，对跨学科方法的需求比以往任何时候都更加强烈。细胞生物学方法已经取得进展，达成机制理解是至关重要的，而生物学限制在阐述理论和解释体外数据方面发挥着关键作用。除了考察体外折叠和分子伴侣机制的最新进展外，本次会议还将重点介绍蛋白质折叠和错误折叠机制的最新进展，以及与蛋白质合成、通过细胞和跨膜的分选和转位、大分子组装和蛋白质降解相关的细胞过程。尽管过去的“细胞内蛋白质折叠”会议在强调体外折叠和生物物理主题或体内折叠的细胞方面之间交替进行，但2006年会议的一个新方面将是整合这些主题。常见的主题包括蛋白质构象疾病，包括神经退行性疾病、淀粉样变性疾病和囊性纤维化，以及这些疾病中涉及的蛋白质如何提供对蛋白质质量控制机制的洞察，以及折叠、错误折叠和组装成低聚物和纤维的内在特性。寻找治疗策略需要对多肽链的行为和细胞环境以及对其施加的限制有基本的了解。我们通过这次会议的目标是将物理和生物科学家聚集在一个互动的环境中，促进教育和对方法、实验局限性的理解，并探索共同的兴趣。这次会议规模小，环境孤立，所有与会者都在附近居住和就餐，这促进了最佳的科学交流。会议形式将为非正式讨论提供广泛的场所。海报会议将允许所有参与者与其他调查人员进行科学互动。演讲者中将有年轻的调查人员和妇女，并鼓励他们参加会议。

DESCRIPTION (provided by applicant): We request funding to support the 2006 Federation of American Society of Experimental Biologists (FASEB) Summer Conference on "Protein Folding in the Cell" to be held July 29 to August 3, 2006 in Saxtons River, VT. The proposed conference will be the ninth in a series of highly successful FASEB Conferences on this topic. This meeting has been unique in its goal to bring together cell biologists/geneticists, biophysicists/biochemists, and mathematical modelers to tackle the complex problems of protein folding and misfolding and implications for human disease. This was the concept behind the first conference in 1990, and it remains a unique opportunity for scientists from very diverse backgrounds. Most protein folding conferences consider either the theoretical and in vitro aspects of folding or the role of molecular chaperones and protein folding catalysts in the cell. However, the need for interdisciplinary approaches is stronger than ever. The cell biological approaches have progressed to where reaching a mechanistic understanding is crucial, and biological constraints play a critical role in formulating theory and interpreting in vitro data. In addition to examining recent advances in in vitro folding and molecular chaperone mechanism, this meeting will focus on the latest developments in the mechanisms of protein folding and misfolding, and the related cellular processes of protein synthesis, sorting and translocation through the cell and across membranes, macromolecular assembly, and protein degradation. Whereas past "Protein Folding in the Cell" meetings have alternated between emphases on in vitro folding and biophysical topics or cellular aspects of folding in vivo, a new aspect of the 2006 meeting will be to integrate these topics. Common themes include diseases of protein conformation including neurodegenerative diseases, amyloidoses, and cystic fibrosis and how the proteins implicated in these diseases have provided insights into the protein quality control machinery and on the intrinsic properties of folding, misfolding, and assembly into oligomers and fibrils. Finding therapeutic strategies requires a fundamental understanding of the behavior of the polypeptide chain and the cellular environment and constraints imposed on it. Our goal through this meeting is to bring together physical and biological scientists in an interactive environment, to promote education and understanding of the approaches, experimental limitations and to explore common interests. The small size and isolated setting of this meeting with all participants residing and taking their meals in close proximity promotes optimal scientific exchange. The conference format will provide extensive arenas for informal discussions. Poster sessions will allow all participants to interact scientifically with other investigators. Young investigators and women will be well-represented among speakers and encouraged to attend the conference.