Correlative 3D Cryo-Imaging of Microorganisms for Understanding Anti- Microbial Agents

这项研究将使用时空相关性高的3D冷冻成像来了解单个AMA如何影响细胞(例如过氧化、脂质塑化、与脂质结合的多价阳离子的螯合和小分子通过它的渗透)，并确定快速杀死的最佳组合。Imperial和电子生物成像中心(EBIC)的低温FIB/扫描电子显微镜(SEM)和钻石光源的低温软X射线层析(SXT)/低温结构照明显微镜(SIM)将用于了解AMA的作用模式以及细胞如何提供阻力(如果有的话)。

This research will employ high spatio-temporal correlative 3D cryo-imaging to understand how individual AMA affects the cell {e.g. peroxidation, lipid plasticisation, chelation of multivalent cations bound to the lipids and permeation of small molecules through it) and to identify the best combination for fast kill. Cryo-FIB/SEM/TEM at Imperial and at the electron Bio Imaging Centre (eBIC) and cryo-Soft X-ray Tomography (SXT)/cryo-Structure Illumination Microscopy (SIM) at Diamond Light Source will be employed to understand AMA's mode of action and how the cells provide resistance, if any.