ANTIOXIDANT PROTECTION IN AGE-ASSOCIATED ATHEROSCLEROSIS

描述(来自申请表)：被指导的研究科学家 发展奖将允许Sara G.Carlson博士成功地 过渡到在心血管领域进行独立研究 衰老。由于卡尔森博士在科学训练方面有扎实的基础，所以 有理由认为，在指导研究学期即将结束时，她 将开发出大量与年龄相关的数据，这些数据将形成 独立研究的地位和资金的基础。这样做的目的是 申请是为了使卡尔森博士能够将她目前的努力转向 老龄化研究：研究生涯发展计划由卡尔森博士组成 承担进行这项研究项目的主要角色，选修课程 从事科学诚信工作，参加研讨会，杂志俱乐部， 研讨会和科学会议；阅读和讨论原创研究 关于氧化损伤和动脉粥样硬化的文章；以及与导师和 在老龄化、心血管疾病和细胞凋亡研究方面接受过培训的顾问。 这项研究的目的是：1)确定氧固醇中的具体步骤- 由改变的ROS产生和调节的诱导凋亡通路 培养的血管细胞中的抗氧化剂，以及2)表征与年龄相关的 加速衰老小鼠动脉粥样硬化病变的形成 模型，并探索抗氧化剂补充剂减少的程度 AGE相关动脉粥样硬化病变形成过程中的细胞凋亡。这些 研究将允许候选人检验总体假设，即 抗氧化剂在动脉粥样硬化过程中发挥保护作用 通过抑制ROS介导的细胞凋亡而伴随衰老。

DESCRIPTION (from application): The Mentored Research Scientist Development Award would allow Dr. Sara G. Carlson, PhD, to make a successful transition into conducting independent research in the field of cardiovascular aging. Since Dr. Carlson has a solid foundation in scientific training, it is reasonable to project that toward the end of the mentored research term, she will have developed a significant body of age-related data that will form a basis for independent research status and funding. The objectives of this application are to enable Dr. Carlson to steer her current efforts toward aging research: the research career development plan consists of Dr. Carlson assuming the primary role in conducting this research project, taking course work in scientific integrity, participating in seminars, journal clubs, workshops, and scientific meetings; reading and discussing original research articles in oxidative damage and atherosclerosis; and meeting with mentors and consultants trained in aging, cardiovascular disease, and apoptosis research. The aims of the research are: 1) to identify specific steps in oxysterol- induced apoptotic pathways that are modulated by altered ROS generation and antioxidants in cultured vascular cells, and 2) to characterize age-associated atherosclerotic lesion development in the senescence-accelerated mouse (SAM) model, and explore the extent to which antioxidant supplementation reduces apoptosis during age-associated atherosclerotic lesion formation. These studies will allow the candidate to test the overall hypothesis that antioxidants exert protective effects in the atherosclerotic processes accompanying aging through inhibition of ROS-mediated apoptosis.