CD8+ T Cell Response to Burn Injury

描述（由申请人提供）：候选人：主要研究者的目标是成为一名独立资助的外科科学家，将基础免疫学转化为解决严重烧伤和创伤中最具挑战性的问题。首席研究员有很强的初步记录，完成了N1H-NRSA创伤培训奖学金，并发表了许多科学手稿。该提案旨在取得长期的科学成功，它概述了一个具体的培训计划，其中包括教学工作、技术研讨会、实验室直接监督、伦理和应用基础科学研究的专业发展。环境：UNC-CH拥有强大的学术传统和培养临床科学家的承诺。该学院拥有许多核心设施，包括Lineberger癌症中心和专门为支持临床科学家发展而设计的分子生物学和生物技术项目。Jeffrey a . Frelinger博士，微生物学和免疫学系主任，本提案的导师，有着长期的科学成功记录，并专门致力于首席研究员的科学发展。所有必要的实验室设备，资源，专业知识，以及成功完成该提案所需的办公空间将提供给首席研究员。外科部门绝对致力于首席研究员的科学发展，他坚定地承诺将75%的时间花在这个提案上。研究：本提案的目标是确定CD8+ T细胞对烧伤损伤的反应。CD8+ T细胞越来越被认为是免疫反应的重要调节因子，但对烧伤的反应却知之甚少。初步数据显示，虽然CTL对烧伤损伤的同种异体反应受损，但活化的CD8+ T细胞的表型显示出夸大的促炎反应。这一提议的假设是CD8+ T细胞对烧伤损伤的反应是多效的，这种反应的机制是CD8+ T细胞活化的改变。首席研究员将使用多种先进的细胞免疫学技术来确定烧伤后CD8+ T细胞的表型和功能。此外，我们还将研究CD8+ T细胞活化的机制，特别是宿主环境和细胞因子环境如何影响CD8+ T细胞对烧伤损伤的反应。

DESCRIPTION (provided by applicant): Candidate: The goal of the principal investigator is to become an independently funded surgical scientist who translates basic immunology into solutions for the most challenging problems in severe burns and trauma. The principal investigator has a strong preliminary record, having completed a N1H-NRSA trauma training fellowship and published a number of scientific manuscripts. The proposal, designed for long-term scientific success, outlines a specific training plan that includes didactic work, technical workshops, direct laboratory supervision, ethics, and professional development in applied basic science research. Environment: UNC-CH has a strong academic tradition and a commitment to training clinician scientists. The school has a number of core facilities, including the Lineberger Cancer Center and the Program in Molecular Biology and Biotechnology specifically designed to support the development of clinician scientists. Dr. Jeffrey A. Frelinger, PhD, Chair, Department of Microbiology and Immunology, the mentor for this proposal, has a long track record of scientific success and is committed specifically to the scientific development of the principal investigator. All required laboratory equipment, resources, expertise, and office space necessary for successful completion of this proposal will be available to the principal investigator. The Department of Surgery is absolutely committed to the scientific development of the principal investigator and his firm commitment to spend 75% of his time on this proposal. Research: The goal of this proposal is to determine the CD8+ T cell response to burn injury. CD8+ T cells, increasingly recognized as an important regulator of the immune response, have a poorly understood response to burn injury. Preliminary data reveals that while the CTL alloresponse to burn injury is impaired, the phenotype of activated CD8+ T cells suggests an exaggerated proinflammatory response. The hypothesis of this proposal is that the CD8+ T cell response to burn injury is pleiotropic and the mechanism for this response is altered CD8+ T cell activation. The principal investigator will use a variety of advanced cellular immunologic techniques to determine the phenotype and function of CD8+ T cells after burn injury. In addition, the mechanisms of CD8+ T cell activation will be investigated, specifically how the host environment and the cytokine environment influence the CD8+ T cell response to burn injury.