BAC Library Production and Maintenance

描述（由申请人提供）：目标是为NIH咨询委员会选择的物种准备十倍冗余的细菌人工染色体（BAC）文库，并根据NIH政策传播文库。这些文库将根据科学界对比较基因组学的兴趣进行定制，并用于支持全基因组测序项目。该项目是美国国立卫生研究院和奥克兰儿童医院研究所早先合作协议的延续。该应用程序有四个与制备（BAC）相关的组件：1)文库制备，2)克隆阵列和文库复制，3)表征，以及4)研究与开发，以提高流程效率和BAC文库质量。在第一年，将为具有典型哺乳动物基因组大小的基因组准备四个BAC文库。这相当于“800,000 BAC克隆”的制备能力。在项目的第二年和第三年，BAC建设方法的改进可能会导致吞吐量略有增加。小规模的延续项目没有提供“规模经济”改进的机会。新的文库将补充已经可用于比较基因组分析的BAC克隆收藏的广泛曲目。新的克隆收集将通过一套标准化测试进行分析，包括使用一套基因组标记进行筛选、有限的bac末端测序和插入物大小测定。一旦库通过了质量控制，新的资源将以与主要应用程序一致的格式提供。为了方便人们进入图书馆，建立了一项以用户费用回收成本为基础的筛选服务。预计新的BAC资源将通过将人类基因与越来越多的动物物种进行比较，继续有助于更好地了解人类基因的功能和进化。BAC克隆也将越来越多地用作创建人类疾病动物模型的工具。广泛传播克隆是该项目的一个重要组成部分。大多数图书馆的使用费不足以支付维护费用。因此，只需要最低限度的资金来支持新图书馆的维护和先前项目倡议下产生的图书馆。有关图书馆的信息可通过BACPAC资源网站（http://bacpac.chori.org）获得。

DESCRIPTION (provided by applicant): The goal is to prepare ten-fold redundant Bacterial Artificial Chromosomes (BAC) libraries for species selected by an NIH advisory committee and to disseminate the libraries in accordance with NIH policy. The libraries will be tailored to the scientific community's interest in comparative genomics and be used in support of whole-genome sequencing projects. The project is a continuation of an earlier cooperative agreement between the NIH and the Children's Hospital Oakland Research Institute. The application has four components relevant to preparing (BACs): 1) Library preparation, 2) Clone Arraying & Library Duplication, 3) Characterization, and 4) Research & Development to improve the process efficiency and BAC library quality. During the first year, four BAC libraries will be prepared for genomes having a typical mammalian genome size. This corresponds with an "800,000 BAC clone" preparation capacity. During years two and three of the program, improvements in BAC construction methods may result in slight increases in throughput. The small size of the continuation program does not offer opportunities for "economy of scale" improvements. The new libraries will complement the extensive repertoire of BAC clone collections already available for comparative genome analysis. The new clone collections will be analyzed by a standardized set of tests, including screening with a set of genomic markers, limited BAC-end sequencing and insert size determination. Once the libraries pass the quality controls, the new resources will be made available in a format consistent with major applications. To facilitate access to the BAC libraries, a screening service based on cost recovery by user fees has been established. It is expected that the new BAC resources will continue to contribute to better understanding of human gene function and evolution through comparison of human genes with a growing spectrum of animal species. BAC clones will also increasingly be used as tools to create animal models for human diseases. Widespread dissemination of the clones is an essential component of the project. User fees for most libraries cannot cover the costs of maintenance. Hence, minimal funding is requested in support of the maintenance of the new libraries and those generated under the previous program initiative. Information about libraries is available through the website for BACPAC Resources (http://bacpac.chori.org).