Using ecophysiology to better predict the uptake of chemicals into fish

淡水化学变化很大，例如，H+和钙根据位置的不同变化了1000倍，这主要影响到影响化学物质吸收的鳃和皮肤的化学物质形态和性质。这个机械项目将填补这一知识空白，使生态风险评估能够根据区域条件进行调整。我们的多学科监督团队在生理学、生态毒理学和分析化学方面提供世界一流的专业知识，以支持学生进行这个真正新颖的项目。通过水生动物鳃/皮肤的上皮电势（TEP）影响带电分子的吸收，但受到淡水化学（例如pH、Ca、盐度、DOC）的自然变化的显著影响（例如从-35 mV到+10 mV）。TEP如何影响化学物质的吸收是生态毒理学的一个主要知识缺口，并可能解释个体鱼类和不同研究之间在化学物质吸收方面的巨大不一致性。该学生将在鱼和细胞培养的体内和体外生理实验方面发展专业知识，包括测量鳃间上皮电势（TEP）和一系列物种的化学吸收。我们将提供与分析化学和理解化学形态相关的技能培训，以及统计和建模方法。

Freshwater chemistry varies greatly, e.g. H+ and calcium by >1,000-fold depending on location, which majorly influence chemical speciation and properties of gills and skin that affect chemical uptake. This mechanistic project will fill this knowledge gap to allow ecological risk assessment to be tailored to match regional conditions. Our multidisciplinary supervisory team provides world-class expertise in physiology, ecotoxicology, and analytical chemistry to support the student in this truly novel project. The transepithelial electrical potential (TEP) across aquatic animal gills/skin influences uptake of charged molecules, but is dramatically influenced (e.g. from -35 to +10 mV) by natural variability in freshwater chemistry (e.g. pH, Ca, salinity, DOC). How TEP affects chemical uptake represents a major knowledge gap in ecotoxicology and may explain large inconsistencies in their uptake between individual fish and different studies.The student will develop expertise in in vivo and in vitro physiology experimentation with fish and cell cultures, including measuring transepithelial electrical potential (TEP) across gills and chemical uptake in a range of species. We will provide training in skills relevant to analytical chemistry and understanding chemical speciation, and statistical and modelling methods.