Small Animal Laboratory Core

该计划项目资助的主题主要基于临床前体内 模型来研究与骨骼上的癌症影响有关的分子机制。 每个项目都需要使用动物模型并对其进行仔细评估和监测 动物的骨骼和软组织转移。这里提出的小动物核心将 为每个项目中涉及动物的所有工作提供支持。服务将包括 肿瘤细胞系的维持，涉及小动物的所有程序的管理以及 评估癌症对骨骼影响的最先进方法。这个核心建立在 通过采用新技术，如光学成像， 表达绿色荧光蛋白的肿瘤细胞、MicroSPECT、MicroPET和microCT， 评估肿瘤对骨骼的影响和肿瘤负荷。小的额外力量 动物核心是这些领域公认的专家的参与。技术如 MicroSPECT、MicroPET和MicroCT用于双侧乳腺癌患者的临床诊断 癌症和多发性骨髓瘤，但据我们所知，尚未在小动物中使用 转移性疾病的模型。使用这些技术将使我们能够直接比较 我们的临床前模型和患者疾病之间的关系， 乳腺癌和多发性骨髓瘤的模型。此外，这些技术将建立在这些 我们过去使用的放射照相术和组织形态测定法， 在整个完整动物中的肿瘤负荷和分布的更全面的图片，而 提供更好的分辨率。

The theme of this Program Project Grant is heavily based around the use of preclinical in vivo models to study the molecular mechanisms involved with the effects to cancer on the skeleton. Each project requires the use of animal models and careful assessment and monitoring of those animals for both skeletal and soft tissue metastases. The Small Animal Core proposed here will provide support for all work involving animals within each individual project. Services will include maintenance of tumor cell lines, management of all procedures involving small animals as well as state-of-the art methods for assessing the effects of cancer on the skeleton. This core builds on the previously existing laboratory core by incorporating new techniques such as optical imaging of green fluorescent protein expressing tumor cells, MicroSPECT, MicroPET and microCT for the assessment of tumor effects of the skeleton and tumor burden. An additional strength of the Small Animal Core is the participation of recognized experts in these areas. Techniques such as MicroSPECT, Micro PET and MicroCT are used in clinical diagnostics for patients with both breast cancer and multiple myeloma but to our knowledge have not yet been utilized in small animal models of metastatic disease. Use of these techniques will allow us to make direct comparisons between our preclinical models and disease in patients providing for validation of our preclinical models of breast cancer and multiple myeloma. Furthermore, these techniques will build on those that we have used in the past such as radiography and histomorphometry by allowing us to obtain a much more thorough picture of tumor burden and distribution in the whole intact animal, while providing much better resolution.