CORE--Molecular Resources Core

核心C，即分子资源核心，将为所有四个项目提供分子试剂和服务，并将对完成计划项目中每个提案的具体目标至关重要。分子资源核心的提议是对原有核心C的延续。核心C的总体功能是提供分子试剂和专业知识，包括产生缺失和定点突变并整合到所需的表达载体中，创建微基因构建体，生成腺病毒和逆转录病毒构建体用于转导原代内皮细胞，以及设计和产生适当的基于siRNA的构建体。该核心将由兰德尔·A·斯基德格尔博士领导，并将配备一名全职高级研究专家(Barbara Keith博士)、全职研究专家(TBN)和兼职研究助理(Vidas Dumasias)。将这些努力整合到核心C中将导致在完成每个 项目以及途径和方法的一致性，以便可以比较每个项目的结果。此外，它还将允许严格的质量控制和各种项目使用的分子试剂的一致性。此外，与每个项目将产生自己的分子资源的方案相比，集中式核心C的一个额外且重要的好处将是显著降低费用。核心领导与高级研究专家和项目领导的定期会议将确保分子试剂生成的协调和优先次序，并确保及时向组成部分项目提供所需的试剂。

Core C, the Molecular Resources Core, will provide molecular reagents and services to all four projects and will be crucial to the completion of the specific aims of each proposal in the Program Project. The proposal for a Molecular Resources Core is a continuation of the original Core C. The overall function of Core C is to provide molecular reagents and expertise, including generation of deletion and site-directed mutants and incorporation into the desired expression vectors, creation of minigene constructs, generation of adenoviral and retroviral constructs for transduction of primary endothelial cells and designing and generating appropriate siRNA based constructs. This core will be directed by Dr. Randal A. Skidgel and will be staffed by a full-time Senior Research Specialist (Dr. Barbara Keith), full- time Research Specialist (TBN) and part-time Research Associate (Vidas Dumasias). Consolidating these efforts in Core C will result in increased speed and efficiency in accomplishing the research tasks outlined in each of the projects and uniformity of approaches and methods so that resutts from each project can be compared. In addition, it will allow strict quality control and consistency of molecular reagents to be used by the various projects. Furthermore, an added and important benefit of the centralized Core C will be to markedly decrease expenses compared to a scenario in which each project would generate its own molecular resources. Regular meetings of the Core leader with the Senior Research Specialist and project leaders will assure coordination and prioritization of the generation of molecular reagents and assure that the desired reagents are provided in a timely manner to the component projects.