Mouse Breeding and Husbandry

小鼠育种核心(核心C)将产生、安置和维护最终繁育者，以获得项目资助(PPG)所有项目所需的单基因和组合基因靶向和转基因小鼠。一旦获得这些动物，这些动物将被移交给PPG项目的个人调查人员进行大规模繁殖，以产生所需数量的动物，以进行拟议的研究。动物综合体由兽医和委员会认证的兽医病理学家马克·A·萨考博士领导，他制定了有关动物护理和繁殖的政策。一名副主任负责管理对十名技术人员的日常监督，这些技术人员负责照顾动物并建立所要求的繁育。其中三个人被分配到W.M.凯克转基因研究中心的动物中，该中心将用于目前的PPG的调查。这一核心的责任将是产生适当的小鼠，当研究人员饲养这些小鼠时，将导致获得研究所需的特定动物的高统计概率。考虑到这些项目中最终动物的遗传菌株的复杂性，具有较低可能性的菌株 产生最终的动物将是首先需要的。核心C将负责后一项任务。这个核心的目标是至少产生：1)X[+/-]或X[-/-](当这种基因的小鼠能够存活和繁殖时)和X+/-/Y-/-(其中Y是第二个基因)或X-/-/Y-/-用于大规模繁殖以产生Castellino项目所需的动物；2)APC[Min+/-o]/X+/-(或X-/-，如果它们存活并繁殖)小鼠，用于Ploplis项目的大规模繁殖；3)为罗森项目产生足够数量的WT和FX+/-育种者。

The Mouse Breeding Core (Core C) will generate, house, and maintain the final breeders needed to obtain the single and combined gene targeted and transgenic mice needed for all projects of the Program Project Grant (PPG). Once obtained, these animals will be turned over to individual investigators of the projects of the PPG for large-scale breedings to generate the required numbers of animals for the studies proposed. The animal complex is directed by a veterinarian and board certified veterinary pathologist, Dr. Mark A. Suckow, who establishes the policies regarding animal care and breeding. An associate director manages day-to-day supervision of the ten technologists who care for the animals and establish the requested breedings. Three of these latter individuals are assigned to animals of the W.M. Keck Center for Transgene Research that will be used in the investigations of the current PPG. The responsibilities of this core will be to generate appropriate mice that when bred by investigators will lead to a high statistical probability of obtaining the specific animals needed for the studies. Given the complexities of the genetic strains of the final animals in these projects, strains with low probabilities of generating the final animals will be first required. Core C will assume responsibility for this latter task. The aims of this core are to generate at a minimum: 1) X[+/-] or X[-/-] (when mice with this genotype are able to survive and breed) mice and X+/-/Y-/- (where Y is a second gene) or X-/-/Y-/- for large-scale breedings to generate animals required for Project by Castellino; 2) APC[Min+/-o]/X+/- (or X-/-, if they survive and breed) mice for large-scale breedings for Project by Ploplis; and 3) to generate sufficient numbers of WT and FX+/- breeders for Project by Rosen.