Ultrafast SBS Method for Large-Scale Human Resequencing

描述（由申请人提供）：在过去的六个月里，我们在开发生物活性可逆终止剂方面取得了实质性进展。一类新的修饰核苷的发现为在不久的将来开发四色可逆终止剂组奠定了基础。尽管如此，在有机合成、化合物建模和UV脱保护方面仍需要大量工作，以将该技术从可行性阶段推向实际应用。我们正在申请补充资金，以加快我们开发超快速DNA测序战略的进展。在这里，我们介绍了我们的第一个染料标记的可逆终止子和逐步测序通过均聚物重复的发展，我们最近的进展。提出了额外的研究目标，以在未来12-18个月内提供四色可逆终止子核苷酸组，用于实施循环可逆终止（CRT）测序平台。

DESCRIPTION (provided by applicant): Over the past six months, we have made substantial progress towards development of biologically-active reversible terminators. The discovery of a new class of modified nucleosides has established the foundation for development of a four-color reversible terminator set in the near future. Still, tremendous work is required in organic synthesis, compound modeling, and UV deprotection to move the technology from the stage of feasibility to practical application. We are requesting supplemental funding to accelerate our progress for developing the ultra-fast DNA sequencing strategy. Here, we present our recent progress on the development of our first dye-labeled, reversible terminator and step-wise sequencing through a homopolymer repeat. Additional research goals are proposed to deliver a four-color, reversible terminator nucleotide set within the next 12-18 months for implementation of the cyclic reversible termination (CRT) sequencing platform.