Community metaproteomics to establish functional responses within the oral microbiome

健康的口腔微生物群对维持口腔系统健康至关重要。口腔疾病花费了NHS数十亿英镑，而口腔保健市场也价值数十亿英镑。然而，对口服产品如何影响口腔微生物群的组成和功能的了解还很薄弱。我们的项目将使用尖端的宏蛋白质组学技术来确定微生物组对常用口腔产品添加剂的功能变化。这将与快速纳米孔16microbiome测序相结合，最初将重点放在氯雷己定（CHX）和GSK开发的一种新活性药物（安置工作）上。CHX是日常漱口水中常见的抗菌药物，持续使用会改变微生物群并影响血压调节化合物。2022条目WR BBSRC DTP CASE表格a - final 20121-07-16第2页15可访问的方式，以吸引广泛的申请人。亚硝酸盐)。此外，使用CHX可诱导某些细菌对抗生素产生耐药性。我们的团队，Stafford + GSK，已经开发了体外微生物群落生长技术，以及口服特异性16S纳米孔微生物分析。我们还关注了这些社区的毒力决定因素（如蛋白酶/糖苷酶）。所开发的数据和技术将允许gsk -消费者医疗保健未来活动的改进和设计

A healthy oral microbiome is essential to maintaining oraland systemic health. Oral diseases cost the NHS £billions while the oralhealthcare market is also worth £billions. However, understanding how oralproducts affect the composition and function of the oral microbiota is weak.Our project will use cutting-edge metaproteomics techniques to establishfunctional changes in the microbiome in response to commonly used oralproduct additives. This will be in combination with rapid nanopore 16Smicrobiome sequencing, and focus initially on Chlorehexidine (CHX) plus anovel active developed at GSK (placement work).CHX is a common antimicrobial in daily mouthwashes, with continued usagealtering the microbiome and affecting blood-pressure modifying compounds2022 entry WR BBSRC DTP CASE Form A-FINAL 2021-07-16 Page 2 of 15accessible manner to appeal to a widerange of applicants.(e.g. nitrite). In addition, CHX use can induce resistance to antibiotics insome bacteria.Our team, Stafford + GSK, have developed techniques for growth ofmicrobial communities in vitro, and oral specific 16S Nanopore microbiomeanalyses. We also focussed on virulence determinants (e.g.protease/glycosidase) in these communities. The data and techniquesdeveloped will allow improvement and design of future actives byGSK-consumer healthcare