Identifying Novel Protein Lysine Methyltransferases

描述(申请人提供)：核糖体和核糖体相关蛋白的赖氨酸甲基化在调节翻译过程中的作用与其在调节转录等其他过程中的作用相比，人们知之甚少。这项建议的目的是在酿酒酵母中鉴定可能参与调节翻译的新的蛋白质赖氨酸甲基转移酶。这将通过分析其中假定包含SET结构域的基因已被删除的敲除菌株来完成。SET结构域甲基转移酶是赖氨酸甲基转移酶，已被证明可以在翻译后修饰细胞色素c、Rubisco和组蛋白等蛋白质。在蛋白质家族数据库的搜索过程中，发现了7个编码赖氨酸甲基转移酶的基因，其中一个我已经确定编码核糖体蛋白赖氨酸甲基转移酶，现在我称之为RKM1。这项提议的目的之一将是确定与RKM1具有最接近同源性的其余基因是否也编码核糖体蛋白赖氨酸甲基转移酶。一旦底物被确定，我计划确定甲基转移酶在调节其底物和随后的翻译中所起的作用。

DESCRIPTION (provided by applicant): The role that lysine methylation of ribosomal and ribosomal-associated proteins plays in regulating the process of translation is poorly understood compared to its role in regulating other processes such as transcription. The purpose of this proposal is to identify novel protein lysine methyltransferases in Saccharomyces cerevisiae that may be involved in regulating translation. This will be accomplished by analyzing knockout strains in which putative SET domain-containing genes have been deleted. SET domain-containing methyltransferases are lysine methyltransferases that have been shown to post-translationally modify proteins such as cytochrome c, Rubisco, and histones. During a search of the Protein Family Database, seven genes encoding putative lysine methyltransferases were found, one of which I have already determined to encode a ribosomal protein lysine methyltransferase, which I now refer to as RKM1. One aim of this proposal will be to determine whether the remaining genes that have the closest homology to RKM1 also encode ribosomal protein lysine methyltransferases. Once the substrates are identified I plan to determine the role the methyltransferase plays in the regulation of its substrate and subsequently translation.