MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

描述（由申请人提供）：调节人眼流出设施的生物力学因素。眼压升高是青光眼视神经损伤的主要危险因素。本研究要验证的中心假设是IOP的周期性变化会影响通过常规流出通道影响流体流动的调节机制。实验采用死后人眼前段灌注模型进行。具体目的如下：1)研究水分泌的昼夜振荡影响常规流出设施的机制。2)探讨脉动性眼压对常规流出设施调节机制的影响。为了这两个目的，对照和实验组将随着时间的推移进行比较，以观察这些生理应激源如何影响以下方面：-流出设施/lOP， -小梁网细胞离体mRNA水平，-常规流出细胞的蛋白质分泌，以及-施莱姆管内壁的孔隙密度。本实验将探讨这两种生理上相关的机械应激源对前段灌注的生理、形态学和生化影响

DESCRIPTION (provided by applicant): Biomechanical factors that regulate outflow facility in human eyes. Elevated intraocular pressure (IOP) is the primary risk factor for optic nerve damage in glaucoma. The central hypothesis to be tested within this research is that periodic changes in IOP impact regulatory mechanisms that affect fluid flow through the conventional outflow pathway. Experiments will be performed using the anterior segment perfusion model using post mortem human eyes. The specific aims are as follows: 1) To examine mechanisms by which diurnal oscillations in aqueous secretion affect conventional outflow facility. 2) To investigate how pulsatile intraocular pressure influences mechanisms that regulate conventional outflow facility. For both aims, control and experimental groups will be compared over time, to observe how these physiological stressors affect the following endopints: - Outflow facility/lOP, - mRNA levels of trabecular meshwork cells ex-vivo, - protein secretion by conventional outflow cells, and - pore density in the inner wall of Schlemm's canal. The experiments outlined in this proposal will examine the physiological, morphological, and biochemical effects of these two physiologically relevant mechanical stressors on the anterior segment perfusion