ACTIVITY 1: MOLECULAR NEUROBIOLOGY UNIT

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这项建议的长期目标是扩大神经生物学研究所研究人员进行神经生物学系统基因表达调节研究的能力。这一扩张的主旨是在研究所建立一个分子神经生物学单位。具体而言，该提案的目的是：1）获得开展此类研究所需的设备，2）为一名高级博士后研究员提供工资支持。所要求的设备和人员被用于扩展常驻科学家正在进行的以下项目：1）龙虾运动系统中A电流多样性的分子机制，2）心脏钠通道蛋白激酶A调节的分子决定因素，3）在神经元解剖和突触连接规范中的作用，4）小龙虾肌调节蛋白神经肽：前体组织和基因表达，5）胺受体基因表达和地位的优势层次的热带对虾，和6）基因表达的变化，诱导碱性成纤维细胞生长因子视神经损伤后。增设一个分子神经生物学单位，研究特定神经蛋白的结构、功能或表达，将大大提高研究所的总体研究能力和科学培训机会。虽然这些研究本质上是基础性的，但它们涉及对理解影响波多黎各人口的神经系统疾病至关重要的重要分子机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The long-term objective of this proposal was to expand the capability of investigators at the Institute of Neurobiology for carrying out research on the regulation of gene expression in neurobiological systems. The thrust of this expansion was to develop a molecular neurobiology unit at the Institute. Specifically, the aims of this proposal were to : 1) obtain the equipment required for carrying out this kind of research, and 2) provide salary support for one senior postdoctoral level investigator. The requested euipment and personnel were used to expand the following on-going projects by resident scientists: 1) molecular mechanisms that underlie A-current diversity in a lobster motor system, 2) molecular determinants of protein kinase A modulation of heart sodium channels, 3) role of engrailed paralogues in specification of neuronal anatomy and synaptic connections, 4) the crayfish myomodulin neuropeptides: precursor organization and gene expression, 5) amine receptor gene expression and status in the dominance hierarchies of a tropical prawn, and 6) changes in gene expression induced by basic fibroblast growth factor after optic nerve injury. The addition of a molecular neurobiology unit to study the structure, function or expression of specific neural proteins would significantly enhance the overall research capacity and opportunities for scientific training at the Institute. Although these studies are basic in nature, they addressed important molecular mechanisms that are critical for understanding neurological conditions affecting the Puerto Rican population