HIV AND SUBSTANCE ABUSE LABORATORY CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。这一组成部分的目标是建立一个核心实验室，配备必要的人力和物质资源，以支持艾滋病毒/艾滋病和滥用药物领域的研究。在今后五年中，将向研究人员提供专门设施，以便在免疫学、滥用药物、艾滋病毒/艾滋病和有关传染病等领域进行科学研究。将开发一个体外系统，在受控的实验室条件下测试成瘾相关药物及其相互作用对艾滋病毒复制和免疫反应的影响。核心实验室将为研究人员提供核酸检测、病毒基因分型、流式细胞术表型、淋巴细胞增殖、细胞毒和非细胞毒活性、细胞因子和趋化因子测定以及滥用药物定量等检测。将继续向用户提供顾问和导师，以有效地发展其项目，并促进与外部研究人员的合作。此外，要求支持改进标本库，实验室信息系统，与Data Core的电子通信，并遵守HIPAA和IRB法规。此外，还描述了rcmi支持的基础设施的制度化工作。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this component is to establish a core laboratory with the needed human and physical resources to support research studies in the areas of HIV/AIDS and substances of abuse. For the next five years, specialized facilities will be made available to researchers for scientific studies in fields of immunology, drugs of abuse, HIV/AIDS and related infectious diseases. An in vitro system will be developed to test for the effect of addiction-related drugs and their interaction on HIV replication and the immune response under controlled laboratory conditions. The core lab will provide researchers with assays for nucleic acids detection, virus genotyping, flow-cytometric phenotyping, lymphocyte proliferation, cytotoxic and non-cytotoxic activity, cytokine and chemokine determinations, and drugs of abuse quantification. Consultants and mentors will continue to be available to users for the effective development of their projects and foster collaboration with external researchers. Moreover, support is requested to improve specimen repository, laboratory information system, electronic communication with Data Core, and compliance with HIPAA and IRB regulations. Furthermore, an institutionalizing effort of RCMI-supported infrastructure is described.