CORE E: PROTEOMICS CORE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。明确目标1。为蛋白质组学中心招募并聘请一位副主任。将招募具有蛋白质质谱学经验的博士级科学家，并聘请他来监督和操作蛋白质组学核心设施。私家侦探和项目总监之间的讨论产生了一项建议，即Cajal研究所决定生物系是否有兴趣雇用一名专门从事蛋白质组学的高级教员，以加强蛋白质组学核心设施的运作。全体教员一致投票赞成这项建议。今年夏天将成立一个招聘委员会，从2005年秋季开始招聘。具体目标#4。采购激光捕获显微解剖设备。将获得激光捕获显微解剖设备，用于分析组织内选定细胞中的蛋白质。安排了设备演示，对激光捕获显微解剖显微镜进行了实际评估。为期一天的研讨会由徕卡仪器和分子机器提供。生物系的所有教师和研究生都被邀请了。大约有35名教师和学生参加了这些讲习班。购买工具的选择将在下一个奖助金财政年度的早期完成。具体目标5。购买辅助蛋白质组学设备。二维凝胶电泳和蛋白质斑点切除设备将获得高分辨率的蛋白质分离。通过与Bio-Rad和GE （Amersham）销售代表的讨论，对定点切割设备进行了评估。选择并订购了Bio-Rad EXQuest点切割机，以及成像设备和图像分析软件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific aim #1. Recruit and hire an associate director for the Proteomics Core facility. A Ph.D. level scientist with experience in protein mass spectrometry will be recruited and hired to supervise and operate the Proteomics Core Facility. Discussions between the P.I. and the Program Director resulted in a proposal that the Cajal Institute determine the Biology Faculty interest in hiring a senior level faculty member specializing in proteomics to enhance the operation of the Proteomics Core Facility. The Faculty unanimously voted in favor of this proposal. A search committee will be formed over the summer, with recruiting beginning in Fall, 2005. Specific aim #4. Purchase laser capture microdissection equipment. Laser capture microdissection equipment will be obtained for analyzing proteins in selected cells within a tissue. Equipment demonstrations were arranged to do hands-on evaluation of laser capture microdissection microscopes. One-day workshops were presented by Leica Instruments and by Molecular Machines. All faculty and graduate students in the Biology Department were invited. The workshops were attended by about 35 faculty and students. Selection of an instrument to purchase will be completed early in the next grant fiscal year. Specific aim #5. Purchase accessory proteomics equipment. Equipment for two-dimensional gel electrophoresis and protein spot excision will be obtained for high resolution protein separations. Spot cutter equipment was evaluated through discussions with Bio-Rad and GE (Amersham) sales representatives. A Bio-Rad EXQuest Spot Cutter was selected and ordered, along with imaging equipment and image analysis software.