ANALYTIC CYTOLOGY CORE FACILITY

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。德克萨斯大学埃尔帕索分校提议继续支持分析细胞学核心设施，这是一个多用户研究设施，致力于协助教职员工和学生设计协议，使用蔡司EM-10透射电子显微镜，蔡司Axioskop epifluorescence显微镜，图像分析和图像处理，以及最近收购的徕卡Ultracut R超低温显微镜。这使我们能够使用冷冻免疫标记技术。有16个学院的教职员工和学生利用该设施进行项目。我们预计，这个核心设施将对我们提供病毒学和生理学职位的新教员至关重要。本实验室提供的其他设备包括带闪点捕获卡的Pulnix 1001模拟/数字相机，使我们能够将数字图像发送到带有Image Pro软件的Dell Optiplex GX110计算机。这些图像可以在柯达染料升华打印机上打印。这为我们提供了一个数码暗室。Pelco固定微波减少了固定所需的时间。Sorvall临界点干燥装置用于另一个实验室利用扫描电镜的项目。蔡司Axioskop配备了35毫米相机、Optronics数码相机、OPTIMAS软件、Gateway电脑、惠普扫描仪、尼康35毫米扫描仪和泰克Phaser 740彩色激光打印机。定制的暗室设施也可用。该设施将配备一名全职主管/技术人员，负责监督用户，监督和及时检查设备，准备化学溶液，维修超显微仪和暗室设备，维护设备日志，执行安全法规，处理废弃化学品，订购用品和设备，监督新设备的安装，并维护库存。将向核心主任提供部分薪金，以便持续监督这些活动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The University of Texas at El Paso proposes to continue support for the Analytical Cytology Core Facility, a multi-user research facility dedicated to assisting faculty, staff and students in the design of protocols, the use of the Zeiss EM-10 transmission electron microscope, the Zeiss Axioskop epifluorescence microscope, image analysis and image processing, as well as the recently acquired Leica Ultracut R ultramicrotome with a cryostage. This enables us to use cryoimmunolableling techniques. There are sixteen faculty whose staff and students have projects utilizing this facility. We anticipate that this core facility will be essential to new faculty to whom we have offered positions in virology and physiology. Additional equipment available in this laboratory includes a Pulnix 1001 analog/digital camera with a Flash point capture card which enables us to send digital images to a Dell Optiplex GX110 computer with Image Pro software. These images can be printed on a KODAK dye sublimation printer . This provides us with a digital darkroom. A Pelco fixation microwave reduces the amount of time needed for fixations. A Sorvall critical point drying apparatus is used for projects utilizing SEM in another laboratory. The Zeiss Axioskop is equipped with a 35 mm camera, an Optronics digital camera, OPTIMAS software, a Gateway computer, a Hewlett Packard scanner, a Nikon 35 mm scanner and a Tektronix Phaser 740 color laser printer. Custom darkroom facilities are also available. This facility will be staffed full-time by a supervisor/technician responsible for supervising users, oversight and timely inspection of equipment, preparing chemical solutions, repairing ultramicrotomes and darkroom equipment, maintaining log books for the equipment, enforcing safety regulations, disposing of waste chemicals, ordering supplies and equipment, supervising the installation of new equipment, and maintaining the inventory. Partial salary will be provided to the core director for ongoing oversight of these activiites.