Metabolic control of immune responses

在免疫细胞中，代谢途径和免疫途径是紧密联系和交叉调节的。虽然传统上认为脂质是细胞膜的静止成分，但近年来发现，脂质代谢和细胞脂质状态在调节免疫细胞的激活和功能方面发挥着核心作用。因此，在感染、炎症性疾病和肿瘤进展过程中，细胞脂代谢在控制免疫反应的结果方面是必不可少的。在这种背景下，控制脂代谢可以调节免疫细胞功能，有利于控制各种疾病的免疫反应。尽管如此，我们对这一代谢-炎症循环的了解仍然有限，需要进一步了解才能挖掘其治疗潜力。项目目标：本项目旨在揭示细胞代谢控制免疫细胞激活和功能的机制。目标：第1/2年。我们将结合下一代测序和脂质组学来确定淋巴细胞代谢和免疫途径之间的分子联系。我们将利用体外培养和细胞和分子生物学技术来操纵脂质代谢途径，并确定它们对免疫反应的影响。我们将使用转基因小鼠和体内疫苗接种/疾病模型来揭示细胞代谢和免疫细胞激活之间的联系

The metabolic and immune pathways are strongly interconnected and cross-regulated in immune cells. While lipids have been traditionally regarded as quiescent components of cell membranes, in recent years it has become clear that lipid metabolism and the cellular lipid status play central roles in the regulation of immune cell activation and function. As a result, cellular lipid metabolism is essential in controlling the outcome of immune responses during infection, inflammatory diseases and tumour progression. In this context, manipulating lipid metabolism can regulate immune cell function and beneficially control immune responses in a variety of diseases. Nonetheless, our knowledge of this metabolic-inflammatory circuit is still limited, and further understanding is needed to exploit its therapeutic potential. Project Aim: This project intends to uncover the mechanisms by which cellular metabolism controls immune cell activation and function Objectives: Year 1/2. We will use a combination of next-generation sequencing and lipidomics to identify the molecular links between metabolic and immune pathways in lymphocytes Year 2/3. We will take advantage of in vitro cultures and cellular and molecular biology techniques to manipulate lipid metabolism pathways and determine their effect on immune responses Year 3/4. We will use transgenic mice and in vivo models of vaccination/disease to uncover the link between cellular metabolism and immune cell activation