Multi-label Molecular FLIM of Breast Cancer

描述(申请人提供)：几个研究小组已经证明了使用靶向荧光造影剂对乳腺癌进行光学分子成像；然而，这些应用通常涉及单一造影剂的成像。这项研究的目的是实现多个外源性癌症标志物的非侵入性多标记光学分子成像，使用荧光寿命成像(FLIM)来区分发射光谱重叠的多个造影剂。多标记薄膜在分子成像中的应用使更多的造影剂在体内同时成像成为可能，这对人类乳腺癌的研究、诊断和预后具有重要的意义。具体目的1.构建多标记膜的模型分子体系，并论证该方法在溶液中的可行性。具体目的II.展示造影剂在细胞培养中对多个分子乳腺癌标志物的选择性靶向，并展示与分子靶点结合后的多标记膜。在小鼠和人乳腺肿瘤小鼠体内实现AIM II的目标。

DESCRIPTION (provided by applicant): Optical molecular imaging of breast cancer using targeted fluorescent contrast agents has been demonstrated by several research groups; however, these applications generally involve imaging of single contrast agents. The objective of the proposed research is to enable non-invasive multi-label optical molecular imaging of multiple exogenous cancer markers, using fluorescence lifetime imaging (FLIM) to discriminate multiple contrast agents with overlapping emission spectra. The use of multi-label FLIM in molecular imaging has the potential to enable simultaneous imaging of many more contrast agents in vivo, which has important implications in the study, diagnosis, and prognosis of human breast cancer. Specific Aim I. Construct a model molecular system for multi-label FLIM and demonstrate feasibility of the approach in solution. Specific Aim II. Demonstrate selective targeting of contrast agents to multiple molecular breast cancer markers in cell culture, and demonstrate multi-label FLIM of the agents after binding with molecular targets. Specific Aim III. Perform the goals of Aim II in vivo in murine and human mammary tumors in the mouse.