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Multi-label Molecular FLIM of Breast Cancer

乳腺癌多标记分子FLIM

基本信息

批准号：
7484163
负责人：
JONATHAN QUINCY BROWN
金额：
$ 5.04万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-01 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7484163
关键词：
Attention Binding Biological Markers Breast Cancer Model Breast Carcinoma Cells Contrast Media Cultured Cells Detection Diagnosis Diagnostic Discrimination Epidermal Growth Factor Receptor Epithelial Event Excision Exhibits Fatty acid glycerol esters Fellowship Fluorescence Goals Growth Factor Human Image Imagery Individual Injection of therapeutic agent Invasive Investigation Label Life MCF7 cell Malignant Neoplasms Mammary Neoplasms Metals Microscopic Microscopy Modality Modeling Molecular Molecular Models Molecular Target Monoclonal Antibodies Mus Names Nude Mice Numbers Optics Primary Neoplasm Protein Overexpression Research Solutions System Techniques Technology Time Tissues Transglutaminases Tumor Cell Line base fluorescence imaging fluorophore in vivo malignant breast neoplasm molecular imaging monolayer nanoparticle nanoshell outcome forecast prognostic quantum receptor function time use tissue/cell culture tool tumor

项目摘要

DESCRIPTION (provided by applicant): Optical molecular imaging of breast cancer using targeted fluorescent contrast agents has been demonstrated by several research groups; however, these applications generally involve imaging of single contrast agents. The objective of the proposed research is to enable non-invasive multi-label optical molecular imaging of multiple exogenous cancer markers, using fluorescence lifetime imaging (FLIM) to discriminate multiple contrast agents with overlapping emission spectra. The use of multi-label FLIM in molecular imaging has the potential to enable simultaneous imaging of many more contrast agents in vivo, which has important implications in the study, diagnosis, and prognosis of human breast cancer. Specific Aim I. Construct a model molecular system for multi-label FLIM and demonstrate feasibility of the approach in solution. Specific Aim II. Demonstrate selective targeting of contrast agents to multiple molecular breast cancer markers in cell culture, and demonstrate multi-label FLIM of the agents after binding with molecular targets. Specific Aim III. Perform the goals of Aim II in vivo in murine and human mammary tumors in the mouse.

描述（由申请人提供）：使用靶向荧光造影剂的乳腺癌光学分子成像已被几个研究小组证明;然而，这些应用通常涉及单一造影剂的成像。拟议研究的目的是实现多种外源性癌症标志物的非侵入性多标记光学分子成像，使用荧光寿命成像（FLIM）来区分具有重叠发射光谱的多种造影剂。在分子成像中使用多标记FLIM具有使体内更多造影剂同时成像的潜力，这在人类乳腺癌的研究、诊断和预后中具有重要意义。具体目标一。构建多标记FLIM的模型分子系统，并证明该方法在溶液中的可行性。具体目标二。证明造影剂选择性靶向细胞培养中的多种分子乳腺癌标志物，并证明造影剂与分子靶点结合后的多标记FLIM。具体目标三。在小鼠和人类乳腺肿瘤中体内实现Aim II的目标。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Optical redox ratio differentiates breast cancer cell lines based on estrogen receptor status.

DOI：
10.1158/0008-5472.can-09-2572
发表时间：
2010-06-01
期刊：
Cancer research
影响因子：
11.2
作者：
Ostrander JH;McMahon CM;Lem S;Millon SR;Brown JQ;Seewaldt VL;Ramanujam N
通讯作者：
Ramanujam N

Optical assessment of tumor resection margins in the breast.

DOI：
10.1109/jstqe.2009.2033257
发表时间：
2010-03-01
期刊：
IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society
影响因子：
0
作者：
Brown JQ;Bydlon TM;Richards LM;Yu B;Kennedy SA;Geradts J;Wilke LG;Junker M;Gallagher J;Barry W;Ramanujam N
通讯作者：
Ramanujam N