LTQ MS for High Sensitivity (Phospho) Proteomics

描述(由申请人提供)：13名NIH资助的研究人员申请资金用于芬兰LTQ线性离子陷阱MS，该MS配备完整的Dionex终极纳米LC系统、Nano-ESI源和带有BioWorks/TurboSEQUEST/SALSA的数据分析工作站，用于高灵敏度蛋白质组学和翻译后修饰分析。与我们目前的LCQ DECA相比，LTQ的灵敏度增加了10倍，实现拟议研究目标的潜力将大大增强。在大奥尔巴尼地区不存在其他LTQ，也没有任何具有MS/MS灵敏度的蛋白质组学或LC-MS/MS/MS多肽磷酸化分析能力的仪器。LTQ的授予将允许对许多项目进行蛋白质组学分析，这些项目远远超出了我们目前的敏感性限制。具体研究领域包括：人类呼吸道细胞色素P450酶的调节/修饰；导致不孕不育的人卵巢自身免疫抗原的鉴定；结核分枝杆菌和鼠疫耶尔森菌生物学；金属诱导的免疫病理学；以及人类溶酶体蛋白表达和甲氨蝶呤耐药性。翻译后修饰和磷酸化确定项目包括：Spisula来源的中心体复杂蛋白；动力蛋白；人类血管平滑肌蛋白；人类病原体齿密螺旋体的运动蛋白；以及结肠癌中的生长因子和信号转导受体。 目前，所有列出的联合研究人员都与沃兹沃斯质谱学/蛋白质组学核心合作。核心主任(首席调查员)在蛋白质质谱学和多仪器、多用户设施的管理方面拥有丰富的经验。LTQ MS将被整合到这个核心中，该核心拥有与奥尔巴尼医学院、纽约州立大学奥尔巴尼分校、伦斯勒理工学院和罗斯威尔公园的研究人员进行常规分析和研究合作的记录，并将使这些机构的其他NIH资助的研究项目受益。

DESCRIPTION (provided by applicant): Thirteen NIH-funded investigators request funds for a Finnigan LTQ linear ion trap MS, equipped with a complete Dionex UltiMate nano-LC system, a nano- ESI source, and a data analysis workstation with BioWorks/TurboSEQUEST/SALSA, for high sensitivity proteomics and post-translational modification analyses. The potential to achieve proposed research goals will be substantially enhanced by the 10-fold sensitivity increase of the LTQ compared to our current LCQ DECA. No other LTQ exists in the greater Albany area, nor is there any instrument with the MS/MS sensitivity for proteomics or LC-MS/MS/MS capability for peptide phosphorylation analyses. Award of the LTQ will permit proteomics analyses on many projects that are well beyond our current sensitivity limits. Specific research areas include: regulation/ modification of cytochrome P450 enzymes in the human respiratory tract; identification of human ovarian autoimmune antigens causing infertility; M. tuberculosis and Yersinia pestis biology; metal-induced immunopathology; and human lysosomal protein expression and methotrexate resistance. Post-translational modification and phosphorylation determination projects include: Spisula-derived centrosome complex proteins; the motor protein, dynein; human vascular smooth muscle proteins; motility proteins of the human pathogen, Treponema denticola; and growth factors and signal transduction receptors in colon cancers. Currently, all listed co-investigators collaborate with the Wadsworth Mass Spectrometry/Proteomics Core. The Core Director (the Principal Investigator) has extensive experience in protein mass spectrometry and the management of a multi-instrument, multi-user facility. The LTQ MS will be integrated into this core, which has a record of both routine analyses and research collaborations with investigators at Albany Medical College, SUNY Albany, Rensselaer Polytechnic Institute, and Roswell Park and will benefit other NIH-funded research programs at these institutions.