Linear Ion-Trap /Fourier Transform Mass Spectrometer

描述(由申请人提供)：本提案申请资金购买线性离子陷阱/傅立叶变换质谱仪(Thermo Finnigan LTQ-FT)，以升级犹他大学的质谱学和蛋白质组学核心设施。对复杂蛋白质和修饰的表征是犹他大学NIH资助的80多名研究人员的具体目标的核心。目前，该机构没有能够满足这些调查人员现有的和不断增长的需求的仪器。LTQ-FT仪器是最先进的，将提供超高灵敏度、高质量精度、高分辨率和快速扫描能力，使研究人员能够实现他们具有挑战性的蛋白质组学目标。它还将在生物医学研究的其他领域中有许多应用，如天然产物的结构阐明。许多主要用户已被确定为现有和未来的项目，这些项目将确保立即和持续的满负荷使用。此外，该文书还将提供给其他区域机构的调查人员。 LTQ-FT将被安置在中央质谱学核心设施中，该设施已经运行了25年以上。它将继续由该机构在核心设施框架内进行管理，其中包括由一个监督委员会和一个咨询委员会进行监测。这一工具得到了非常强有力的机构支持。该机构致力于提供最先进的蛋白质组学资源，这将与研究人员建立密切合作的角色。该机构将保证提供资金，以平衡仪器的成本、持续的财政支持、维护、专家质谱学家的全职操作以及蛋白质组学核心设施内的适当空间。作为这一承诺的一部分，该机构最近聘请了一名全职主任，并将聘请更多的博士工作人员为用户操作该仪器。

DESCRIPTION (provided by applicant): This proposal is a request for funds to purchase a linear ion-trap/fourier transform mass spectrometer (Thermo Finnigan LTQ-FT) to upgrade the Mass Spectrometry and Proteomics Core Facility at the University of Utah. Characterization of complex proteins and modifications is central to the specific aims of more than 80 NIH-funded researchers at the University of Utah. Currently, the institution does not have instrumentation capable of meeting the existing and growing needs of these investigators. The LTQ-FT instrument is state-of-the-art and will provide ultra-high sensitivity, high mass accuracy, high resolution, and fast scanning capabilities, enabling researchers to achieve their challenging proteomics goals. It will also have many applications in other areas of biomedical research, such as structure elucidation of natural products. Many major users have been identified with existing and future projects that will ensure immediate and continued full-capacity usage. Also, the instrument will be made available to investigators from other regional institutions. The LTQ-FT will be housed in a central mass spectrometry core facility that has been in operation for more than 25 years. It will continue to be administered by the institution within a core-facilities framework which includes monitoring by an oversight committee and an advisory committee. There is very strong institutional support for this instrument. The institution is committed to providing a state-of-the-art proteomics resource which will build a close-working, collaborative role with investigators. The institution will guarantee funding for the balance of the cost of the instrument, continued financial support, maintenance, full-time operation from expert mass spectrometrists, and appropriate space within the Proteomics Core Facility. As part of this commitment, the institution recently hired a full-time director and will hire additional Ph.D. staff to operate the instrument for users.