The Role of Separase During Cytokinesis in C elegans

描述（由申请人提供）：细胞分裂过程需要各种细胞过程的正确顺序和时间，以确保遗传物质和细胞内容物的忠实传递。进入分裂后期时，姐妹染色单体的凝聚力被一种叫做分离酶的蛋白酶裂解。分离酶最近被证明促进后期纺锤体动力学和胞质分裂伴随着染色体分离在芽殖酵母。本实验室以前的研究在C.优雅从这些分离酶突变蠕虫转移到限制性温度显示几个减数分裂和有丝分裂的缺陷，如染色体不分离，渗透和机械敏感性，胞质分裂失败的胚胎。利用各种遗传学和细胞生物学技术，我打算揭示分离酶在C.优雅通过这一分析，我希望扩展的理解的分子机制，用于时间协调的染色体分离与后期和胞质分裂的开始。

DESCRIPTION (provided by applicant): The process of cell division requires the correct sequence and timing of various cellular processes to ensure faithful transmission of the genetic material and cellular contents. The entry to anaphase occurs upon the cleavage of sister-chromatid cohesion by a protease known as separase. Separase has recently been shown to promote anaphase spindle dynamics and cytokinesis concomitantly with chromosome segregation in budding yeast. Previous studies from our laboratory identified a temperature-sensitive allele of the separase homologue in C. elegans. Embryos from these separase-mutant worms shifted to the restrictive temperature display several meiotic and mitotic defects such as chromosome nondisjunction, osmotic and mechanical sensitivity, and cytokinesis failure. Using various genetic and cell biological techniques, I propose to uncover the mechanisms by which separase contributes to the completion of cytokinesis in the early embryo of C. elegans. Through this analysis, I hope to extend the understanding of the molecular mechanisms utilized for the temporal coordination of chromosome segregation with the onset of anaphase and cytokinesis.