Genomics resources and infrastructure for B. schlosseri

描述(由申请人提供)：本提案的目标是为殖民地原索动物Botryilus schlosseri创建基因组资源和基础设施。斯氏杆菌是一种实验室饲养的、可通过基因操作的生物，其生活史包括许多独特的、可通过实验获得的发育和免疫过程，使其成为各种研究的潜在模式生物。其中包括类似于脊椎动物MHC控制的组织相容性系统，具有在基因决定的等级中寄生相关个体的能力的干细胞，以及两个独立的发育计划--一个是有性的，另一个是无性的--导致形态和功能上相同的身体计划。原脊椎动物代表原始脊椎动物，这种密切的系统发育关系表明，控制Botryllus这些生物现象的基因和调控机制是保守的，与脊椎动物生物学相关。这一建议将创建：完整的施氏杆菌基因组遗传和初始物理图谱；一些标准化和排列的cDNA文库；来自这些标准化文库的4-5,000个克隆的EST数据库，以及转基因和/或瞬时基因表达系统的开发。此外，这些资源还将用于生成初步数据，以便今后提供资金。

DESCRIPTION (provided by applicant): The objective of this proposal is to create the genomic resources and infrastructure for the colonial protochordate, Botryilus schlosseri. B. schlossei is a laboratory reared, genetically manipulable organism with a life history that includes a number of unique and experimentally accessible developmental and immunological processes, making it a potential model organism for a variety of studies. These include a histocompatibility system analogous to that controlled by the MHC in vertebrates, stem cells with the ability to parasitize related individuals in a genetically determined hierarchy, and two separate developmental programs- one sexual and the other asexual-that result in morphologically and functionally identical body plans. The protochordates represent the ancestral vertebrates, and this close phylogenetic relationship suggests that the genes and regulatory mechanisms that control these biological phenomena in Botryllus are Iconserved and relevant to vertebrate biology. This proposal will create: a complete genetic and initial physical map of the B. schlosseri genome; a number of normalized and arrayed cDNA libraries; an EST database of 4-5,000 clones from these normalized libraries, and the development of transgenic and/or transient gene expression systems. In addition, these resources will be used to generate preliminary data for future funding.